Effects of synergistic inhibition on α-glucosidase by phytoalexins in soybeans

Son Hyeong-U; Yoon Eun-Kyeong; Yoo Chi-Yeol; Park Chul-Hong; Bae Myung-Ae; Kim Tae-Ho; Lee Chang Hyung; Ki Won Lee; Hogyun Seo; Kim Kyung-Jin; Lee Sang-Han

doi:10.3390/biom9120828

Effects of synergistic inhibition on α-glucosidase by phytoalexins in soybeans

Son Hyeong-U, Yoon Eun-Kyeong, Yoo Chi-Yeol, Park Chul-Hong, Bae Myung-Ae, Kim Tae-Ho, Lee Chang Hyung, Ki Won Lee, Hogyun Seo, Kim Kyung-Jin, Lee Sang-Han

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed α-glucosidase inhibition kinetics using Michaelis–Menten plots and Lineweaver–Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The K_i values were determined using a Dixon plot as glyceollin, 18.99 μM; genistein, 15.42 μM; luteolin, 16.81 μM; and daidzein, 9.99 μM. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on α-glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI–isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of inhibition.

Original language	English
Article number	828
Journal	Biomolecules
Volume	9
Issue number	12
DOIs	https://doi.org/10.3390/biom9120828
State	Published - Dec 2019

Keywords

Combination index
Daidzein
Enzyme kinetics
Genistein
Glyceollin
Luteolin
Phytoalexins
α-glucosidase inhibitor

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title = "Effects of synergistic inhibition on α-glucosidase by phytoalexins in soybeans",

abstract = "To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed α-glucosidase inhibition kinetics using Michaelis–Menten plots and Lineweaver–Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as glyceollin, 18.99 μM; genistein, 15.42 μM; luteolin, 16.81 μM; and daidzein, 9.99 μM. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on α-glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI–isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of inhibition.",

keywords = "Combination index, Daidzein, Enzyme kinetics, Genistein, Glyceollin, Luteolin, Phytoalexins, α-glucosidase inhibitor",

author = "Son Hyeong-U and Yoon Eun-Kyeong and Yoo Chi-Yeol and Park Chul-Hong and Bae Myung-Ae and Kim Tae-Ho and {Chang Hyung}, Lee and {Won Lee}, Ki and Hogyun Seo and Kim Kyung-Jin and Lee Sang-Han",

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year = "2019",

month = dec,

doi = "10.3390/biom9120828",

language = "English",

volume = "9",

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T1 - Effects of synergistic inhibition on α-glucosidase by phytoalexins in soybeans

AU - Hyeong-U, Son

AU - Eun-Kyeong, Yoon

AU - Chi-Yeol, Yoo

AU - Chul-Hong, Park

AU - Myung-Ae, Bae

AU - Tae-Ho, Kim

AU - Chang Hyung, Lee

AU - Won Lee, Ki

AU - Seo, Hogyun

AU - Kyung-Jin, Kim

AU - Sang-Han, Lee

PY - 2019/12

Y1 - 2019/12

N2 - To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed α-glucosidase inhibition kinetics using Michaelis–Menten plots and Lineweaver–Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as glyceollin, 18.99 μM; genistein, 15.42 μM; luteolin, 16.81 μM; and daidzein, 9.99 μM. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on α-glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI–isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of inhibition.

AB - To determine the mechanism of action of the effects of phytoalexins in soybeans, we analyzed α-glucosidase inhibition kinetics using Michaelis–Menten plots and Lineweaver–Burk plots. The results showed that the type of inhibition with glyceollin was competitive, that of genistein was noncompetitive, that of daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as glyceollin, 18.99 μM; genistein, 15.42 μM; luteolin, 16.81 μM; and daidzein, 9.99 μM. Furthermore, potential synergistic effects between glyceollin and the three polyphenols were investigated. A combination of glyceollin and luteolin at a ratio of 3:7 exhibited synergistic effects on α-glucosidase inhibition, having a combination index (CI) of 0.64244, according to the CI–isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of inhibition.

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KW - Enzyme kinetics

KW - Genistein

KW - Glyceollin

KW - Luteolin

KW - Phytoalexins

KW - α-glucosidase inhibitor

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JO - Biomolecules

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Effects of synergistic inhibition on α-glucosidase by phytoalexins in soybeans

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