Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf

Moonkyeung Choi; Yu Ra Kang; Hyo Don Zu; In Sook Lim; Sung Keun Jung; Yoon Hyuk Chang

doi:10.1007/s12257-019-0259-3

Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf

Moonkyeung Choi, Yu Ra Kang, Hyo Don Zu, In Sook Lim, Sung Keun Jung, Yoon Hyuk Chang

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.

Original language	English
Pages (from-to)	9-15
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	25
Issue number	1
DOIs	https://doi.org/10.1007/s12257-019-0259-3
State	Published - 1 Feb 2020

Keywords

bioactive compounds
flavonoids
food ingredients
natural antioxidants
phenolics

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title = "Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf",

abstract = "We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.",

keywords = "bioactive compounds, flavonoids, food ingredients, natural antioxidants, phenolics",

author = "Moonkyeung Choi and Kang, {Yu Ra} and Zu, {Hyo Don} and Lim, {In Sook} and Jung, {Sung Keun} and Chang, {Yoon Hyuk}",

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AU - Choi, Moonkyeung

AU - Kang, Yu Ra

AU - Zu, Hyo Don

AU - Lim, In Sook

AU - Jung, Sung Keun

AU - Chang, Yoon Hyuk

PY - 2020/2/1

Y1 - 2020/2/1

N2 - We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.

AB - We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.

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Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf

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Keywords

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