Efficient radiolabeling of rutin with ¹²⁵I and biodistribution study of radiolabeled rutin

The purpose of the current research is to synthesize radiolabeled rutin for biodistribution study and SPECT/CT image of rutin. The optimized radiolabeling condition provided ¹²⁵I-labeled rutin with 53.5 % of radiochemical yield. Most of orally administered ¹²⁵I-labeled rutin was initially found in the stomach and small intestine and a portion of the product was then distributed in internal organs. While intravenously injected ¹²⁵I-labeled rutin was accumulated in liver and then a large part of it was transferred to small intestine. The present results provided an efficient radiolabeling method of flavonoid glycoside as well as quantitative organ distribution of rutin.