TY - JOUR
T1 - Electrochemical sandwich-type biosensors for α−1 antitrypsin with carbon nanotubes and alkaline phosphatase labeled antibody-silver nanoparticles
AU - Zhu, Gangbing
AU - Lee, Hye Jin
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/3/15
Y1 - 2017/3/15
N2 - A novel sandwich-type biosensor was developed for the electrochemical detection of α−1 antitrypsin (AAT, a recognized biomarker for Alzheimer's disease). The biosensor was composed of 3, 4, 9, 10-perylene tetracarboxylic acid/carbon nanotubes (PTCA-CNTs) as a sensing platform and alkaline phosphatase-labeled AAT antibody functionalized silver nanoparticles (ALP-AAT Ab-Ag NPs) as a signal enhancer. CNTs offer high surface area and good electrical conductivity. Importantly, Ag NPs could increase the amount of ALP on the sensing surface and the ALP could dephosphorylate 4-amino phenyl phosphate (APP) enzymatically to produce electroactive species 4-aminophenol (AP). For detecting AAT based on the sandwich-type biosensor, the results show that the peak current value of AP using ALP-AAT Ab-Ag NPs as signal enhancer is much higher than that by using ALP-AAT Ab bioconjugate (without Ag NPs), the biosensor exhibited desirable performance for AAT determination with a wide linearity in the range from 0.05 to 20.0 pM and a low detection limit of 0.01 pM. Finally, the developed sensor was successfully applied to the analysis of AAT concentration in serum samples.
AB - A novel sandwich-type biosensor was developed for the electrochemical detection of α−1 antitrypsin (AAT, a recognized biomarker for Alzheimer's disease). The biosensor was composed of 3, 4, 9, 10-perylene tetracarboxylic acid/carbon nanotubes (PTCA-CNTs) as a sensing platform and alkaline phosphatase-labeled AAT antibody functionalized silver nanoparticles (ALP-AAT Ab-Ag NPs) as a signal enhancer. CNTs offer high surface area and good electrical conductivity. Importantly, Ag NPs could increase the amount of ALP on the sensing surface and the ALP could dephosphorylate 4-amino phenyl phosphate (APP) enzymatically to produce electroactive species 4-aminophenol (AP). For detecting AAT based on the sandwich-type biosensor, the results show that the peak current value of AP using ALP-AAT Ab-Ag NPs as signal enhancer is much higher than that by using ALP-AAT Ab bioconjugate (without Ag NPs), the biosensor exhibited desirable performance for AAT determination with a wide linearity in the range from 0.05 to 20.0 pM and a low detection limit of 0.01 pM. Finally, the developed sensor was successfully applied to the analysis of AAT concentration in serum samples.
KW - Alzheimer's disease
KW - Antibody functionalized silver nanoparticles
KW - Electrochemical biosensors
KW - Sandwich assays
KW - α−1 antitrypsin
UR - http://www.scopus.com/inward/record.url?scp=85006288795&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.09.080
DO - 10.1016/j.bios.2016.09.080
M3 - Article
C2 - 27816594
AN - SCOPUS:85006288795
SN - 0956-5663
VL - 89
SP - 959
EP - 963
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -