Electrospun PHBV/collagen composite nanofibrous scaffolds for tissue engineering

Wan Meng, Se Yong Kim, Jiang Yuan, Jung Chul Kim, Oh Hyeong Kwon, Naoki Kawazoe, Guoping Chen, Yoshihiro Ito, Inn Kyu Kang

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

Electrospinning has recently emerged as a leading technique for the formation of nanofibrous structures made of synthetic and natural extracellular matrix components. In this study, nanofibrous scaffolds were obtained by electrospinning a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and type-I collagen in 1,1,1,3,3,3-hexafluoro-2-isopropanol (HIFP). The resulting fibers ranged from 300 to 600 nm in diameter. Their surfaces were characterized by attenuated total reflection Fourier transform infrared spectroscopy, electron spectroscopy for chemical analysis and atomic force microscopy. The PHBV and collagen components of the PHBV/collagen nanofibrous scaffold were biodegraded by PHB depolymerase and a type-I collagenase aqueous solution, respectively. The cell culture experiments indicated that the PHBV/collagen nanofibrous scaffold accelerated the adhesion and growth of NIH3T3 cells more effectively than the PHBV nanofibrous scaffold, thus making the former a good scaffold for tissue engineering.

Original languageEnglish
Pages (from-to)81-94
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume18
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Collagen
  • Electrospinning
  • Nanofibrous scaffold
  • Tissue engineering

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