TY - JOUR
T1 - Enantioseparation on Amino Alcohol-Derived Stationary Phases by Supercritical Fluid Chromatography and High-Performance Liquid Chromatograpy
AU - Kim, Gyuri
AU - Ryoo, Jae Jeong
N1 - Publisher Copyright:
© 2020 Korean Chemical Society, Seoul & Wiley-VCH GmbH
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Previous studies have shown that amino alcohol-derived chiral stationary phases can effectively separate some chiral samples. With the aim at improving the chiral separation, in this study, the composition and type of the mobile phase and the particle size of the stationary phase are changed, and the analytical method is carried out in parallel using high-performance liquid chromatograpy (HPLC) and supercritical fluid chromatography (SFC). By changing the type and composition of the mobile phase and reducing the particle size of the stationary phase, optimal separation and analysis conditions are obtained simultaneously for HPLC and SFC experiments. In particular, for a particle size of 3 μm, the resolution increases for dinitrobenzoyl phenylglycinol and 2,2,2-trifluoro-1-(9-anthryl)ethanol. Moreover, replacing the organic modifier from methanol to isopropanol allows obtaining better results in the SFC experiments of dinitrobenzoyl phenylglycinol, dinitrobenzoyl leucinol, and 2,2,2-trifluoro-1-(9-anthryl)ethanol GTOC,Chiral separation by SFC and HPLC.
AB - Previous studies have shown that amino alcohol-derived chiral stationary phases can effectively separate some chiral samples. With the aim at improving the chiral separation, in this study, the composition and type of the mobile phase and the particle size of the stationary phase are changed, and the analytical method is carried out in parallel using high-performance liquid chromatograpy (HPLC) and supercritical fluid chromatography (SFC). By changing the type and composition of the mobile phase and reducing the particle size of the stationary phase, optimal separation and analysis conditions are obtained simultaneously for HPLC and SFC experiments. In particular, for a particle size of 3 μm, the resolution increases for dinitrobenzoyl phenylglycinol and 2,2,2-trifluoro-1-(9-anthryl)ethanol. Moreover, replacing the organic modifier from methanol to isopropanol allows obtaining better results in the SFC experiments of dinitrobenzoyl phenylglycinol, dinitrobenzoyl leucinol, and 2,2,2-trifluoro-1-(9-anthryl)ethanol GTOC,Chiral separation by SFC and HPLC.
KW - Chiral separation
KW - Chiral stationary phase
KW - HPLC
KW - SFC
UR - http://www.scopus.com/inward/record.url?scp=85091028350&partnerID=8YFLogxK
U2 - 10.1002/bkcs.12094
DO - 10.1002/bkcs.12094
M3 - Article
AN - SCOPUS:85091028350
SN - 0253-2964
VL - 41
SP - 943
EP - 947
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 9
ER -