Engineered extracellular vesicle mimetics from macrophage promotes hair growth in mice and promotes human hair follicle growth

Ramya Lakshmi Rajendran; Prakash Gangadaran; Mi Hee Kwack; Ji Min Oh; Chae Moon Hong; Arunnehru Gopal; Young Kwan Sung; Jaetae Lee; Byeong Cheol Ahn

doi:10.1016/j.yexcr.2021.112887

Engineered extracellular vesicle mimetics from macrophage promotes hair growth in mice and promotes human hair follicle growth

Ramya Lakshmi Rajendran, Prakash Gangadaran, Mi Hee Kwack, Ji Min Oh, Chae Moon Hong, Arunnehru Gopal, Young Kwan Sung, Jaetae Lee, Byeong Cheol Ahn

Department of Medicine

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Recent studies clearly show that cell-derived extracellular vesicles (EVs, including exosomes) can promote hair growth. However, large-scale production of EVs remains a big hurdle. Recently, extracellular vesicle mimetics (EMs) engineered by extrusion through various membranes are emerging as a complementary approach for large-scale production. In this study, to investigate their ability to induce hair growth, we generated macrophage-engineered EMs (MAC-EMs) that activated the human dermal papilla (DP) cells in vitro. MAC-EMs intradermally injected into the skin of C57BL/6 mice were retained for up to 72 h. Microscopy imaging revealed that MAC-EMs were predominately internalized into hair follicles. The MAC-EMs treatment induced hair regrowth in mice and hair shaft elongation in a human hair follicle, suggesting the potential of MAC-EMs as an alternative to EVs to overcome clinical limitation.

Original language	English
Article number	112887
Journal	Experimental Cell Research
Volume	409
Issue number	1
DOIs	https://doi.org/10.1016/j.yexcr.2021.112887
State	Published - 1 Dec 2021

Keywords

Extracellular vesicle mimetics
Hair growth
Macrophage
Mice and human hair follicle

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10.1016/j.yexcr.2021.112887

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title = "Engineered extracellular vesicle mimetics from macrophage promotes hair growth in mice and promotes human hair follicle growth",

abstract = "Recent studies clearly show that cell-derived extracellular vesicles (EVs, including exosomes) can promote hair growth. However, large-scale production of EVs remains a big hurdle. Recently, extracellular vesicle mimetics (EMs) engineered by extrusion through various membranes are emerging as a complementary approach for large-scale production. In this study, to investigate their ability to induce hair growth, we generated macrophage-engineered EMs (MAC-EMs) that activated the human dermal papilla (DP) cells in vitro. MAC-EMs intradermally injected into the skin of C57BL/6 mice were retained for up to 72 h. Microscopy imaging revealed that MAC-EMs were predominately internalized into hair follicles. The MAC-EMs treatment induced hair regrowth in mice and hair shaft elongation in a human hair follicle, suggesting the potential of MAC-EMs as an alternative to EVs to overcome clinical limitation.",

keywords = "Extracellular vesicle mimetics, Hair growth, Macrophage, Mice and human hair follicle",

author = "Rajendran, {Ramya Lakshmi} and Prakash Gangadaran and Kwack, {Mi Hee} and Oh, {Ji Min} and Hong, {Chae Moon} and Arunnehru Gopal and Sung, {Young Kwan} and Jaetae Lee and Ahn, {Byeong Cheol}",

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year = "2021",

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doi = "10.1016/j.yexcr.2021.112887",

language = "English",

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AU - Rajendran, Ramya Lakshmi

AU - Gangadaran, Prakash

AU - Kwack, Mi Hee

AU - Oh, Ji Min

AU - Hong, Chae Moon

AU - Gopal, Arunnehru

AU - Sung, Young Kwan

AU - Lee, Jaetae

AU - Ahn, Byeong Cheol

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Recent studies clearly show that cell-derived extracellular vesicles (EVs, including exosomes) can promote hair growth. However, large-scale production of EVs remains a big hurdle. Recently, extracellular vesicle mimetics (EMs) engineered by extrusion through various membranes are emerging as a complementary approach for large-scale production. In this study, to investigate their ability to induce hair growth, we generated macrophage-engineered EMs (MAC-EMs) that activated the human dermal papilla (DP) cells in vitro. MAC-EMs intradermally injected into the skin of C57BL/6 mice were retained for up to 72 h. Microscopy imaging revealed that MAC-EMs were predominately internalized into hair follicles. The MAC-EMs treatment induced hair regrowth in mice and hair shaft elongation in a human hair follicle, suggesting the potential of MAC-EMs as an alternative to EVs to overcome clinical limitation.

AB - Recent studies clearly show that cell-derived extracellular vesicles (EVs, including exosomes) can promote hair growth. However, large-scale production of EVs remains a big hurdle. Recently, extracellular vesicle mimetics (EMs) engineered by extrusion through various membranes are emerging as a complementary approach for large-scale production. In this study, to investigate their ability to induce hair growth, we generated macrophage-engineered EMs (MAC-EMs) that activated the human dermal papilla (DP) cells in vitro. MAC-EMs intradermally injected into the skin of C57BL/6 mice were retained for up to 72 h. Microscopy imaging revealed that MAC-EMs were predominately internalized into hair follicles. The MAC-EMs treatment induced hair regrowth in mice and hair shaft elongation in a human hair follicle, suggesting the potential of MAC-EMs as an alternative to EVs to overcome clinical limitation.

KW - Extracellular vesicle mimetics

KW - Hair growth

KW - Macrophage

KW - Mice and human hair follicle

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Engineered extracellular vesicle mimetics from macrophage promotes hair growth in mice and promotes human hair follicle growth

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