Enhanced tolerance of transgenic rice overexpressing arabidopsis thaliana nucleoside diphosphate kinase (AtNDPK2) against various environmental stresses

Norvie L. Manigbas; Dong Soo Park; Soo Kwon Park; Sang Ik Han; Hyang Mi Park; Woon Ha Hwang; Sang Min Kim; Hoe Jeong Wang; Hang Won Kang; Doh Hoon Kim; Dae Jin Yun; Gihwan Yi

Enhanced tolerance of transgenic rice overexpressing arabidopsis thaliana nucleoside diphosphate kinase (AtNDPK2) against various environmental stresses

Norvie L. Manigbas, Dong Soo Park, Soo Kwon Park, Sang Ik Han, Hyang Mi Park, Woon Ha Hwang, Sang Min Kim, Hoe Jeong Wang, Hang Won Kang, Doh Hoon Kim, Dae Jin Yun, Gihwan Yi

Department of Farm Management

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Multiple tests to assess different abiotic stresses in rice overexpressing the Arabidopsis thaliana Nucleoside Diphosphate Kinase (AtNDPK2) gene have not been extensively studied. We present the responses of transgenic AtNDPK2 rice lines driven by two different promoters from sweet potato (SWPA2) and cauliflower mosaic virus (CaMV 35S). Transgenic rice lines were evaluated in terms of their tolerance for various abiotic stresses, including cold, drought, ozone, salinity and ultraviolet irradiation. Our results indicate that the transgenic lines were highly tolerant of osmotic stress at 20% polyethylene glycol (PEG) and of salt stress at 100 mM NaCl. The same tolerance was observed with the 5-h continuous UV treatment and ozone. Moderate to weak tolerance for cold and salinity was observed. Our results suggest that the AtNDPK2 gene was responsible for the tolerance for various abiotic stresses in rice.

Original language	English
Pages (from-to)	29-37
Number of pages	9
Journal	Philippine Agricultural Scientist
Volume	94
Issue number	1
State	Published - Mar 2011

Keywords

AtNDPK2
Environmental stresses
Transgenic rice

Cite this

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title = "Enhanced tolerance of transgenic rice overexpressing arabidopsis thaliana nucleoside diphosphate kinase (AtNDPK2) against various environmental stresses",

abstract = "Multiple tests to assess different abiotic stresses in rice overexpressing the Arabidopsis thaliana Nucleoside Diphosphate Kinase (AtNDPK2) gene have not been extensively studied. We present the responses of transgenic AtNDPK2 rice lines driven by two different promoters from sweet potato (SWPA2) and cauliflower mosaic virus (CaMV 35S). Transgenic rice lines were evaluated in terms of their tolerance for various abiotic stresses, including cold, drought, ozone, salinity and ultraviolet irradiation. Our results indicate that the transgenic lines were highly tolerant of osmotic stress at 20% polyethylene glycol (PEG) and of salt stress at 100 mM NaCl. The same tolerance was observed with the 5-h continuous UV treatment and ozone. Moderate to weak tolerance for cold and salinity was observed. Our results suggest that the AtNDPK2 gene was responsible for the tolerance for various abiotic stresses in rice.",

keywords = "AtNDPK2, Environmental stresses, Transgenic rice",

author = "Manigbas, {Norvie L.} and Park, {Dong Soo} and Park, {Soo Kwon} and Han, {Sang Ik} and Park, {Hyang Mi} and Hwang, {Woon Ha} and Kim, {Sang Min} and Wang, {Hoe Jeong} and Kang, {Hang Won} and Kim, {Doh Hoon} and Yun, {Dae Jin} and Gihwan Yi",

year = "2011",

month = mar,

language = "English",

volume = "94",

pages = "29--37",

journal = "Philippine Agricultural Scientist",

issn = "0031-7454",

publisher = "University of the Philippines Los Banos",

number = "1",

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TY - JOUR

T1 - Enhanced tolerance of transgenic rice overexpressing arabidopsis thaliana nucleoside diphosphate kinase (AtNDPK2) against various environmental stresses

AU - Manigbas, Norvie L.

AU - Park, Dong Soo

AU - Park, Soo Kwon

AU - Han, Sang Ik

AU - Park, Hyang Mi

AU - Hwang, Woon Ha

AU - Kim, Sang Min

AU - Wang, Hoe Jeong

AU - Kang, Hang Won

AU - Kim, Doh Hoon

AU - Yun, Dae Jin

AU - Yi, Gihwan

PY - 2011/3

Y1 - 2011/3

N2 - Multiple tests to assess different abiotic stresses in rice overexpressing the Arabidopsis thaliana Nucleoside Diphosphate Kinase (AtNDPK2) gene have not been extensively studied. We present the responses of transgenic AtNDPK2 rice lines driven by two different promoters from sweet potato (SWPA2) and cauliflower mosaic virus (CaMV 35S). Transgenic rice lines were evaluated in terms of their tolerance for various abiotic stresses, including cold, drought, ozone, salinity and ultraviolet irradiation. Our results indicate that the transgenic lines were highly tolerant of osmotic stress at 20% polyethylene glycol (PEG) and of salt stress at 100 mM NaCl. The same tolerance was observed with the 5-h continuous UV treatment and ozone. Moderate to weak tolerance for cold and salinity was observed. Our results suggest that the AtNDPK2 gene was responsible for the tolerance for various abiotic stresses in rice.

AB - Multiple tests to assess different abiotic stresses in rice overexpressing the Arabidopsis thaliana Nucleoside Diphosphate Kinase (AtNDPK2) gene have not been extensively studied. We present the responses of transgenic AtNDPK2 rice lines driven by two different promoters from sweet potato (SWPA2) and cauliflower mosaic virus (CaMV 35S). Transgenic rice lines were evaluated in terms of their tolerance for various abiotic stresses, including cold, drought, ozone, salinity and ultraviolet irradiation. Our results indicate that the transgenic lines were highly tolerant of osmotic stress at 20% polyethylene glycol (PEG) and of salt stress at 100 mM NaCl. The same tolerance was observed with the 5-h continuous UV treatment and ozone. Moderate to weak tolerance for cold and salinity was observed. Our results suggest that the AtNDPK2 gene was responsible for the tolerance for various abiotic stresses in rice.

KW - AtNDPK2

KW - Environmental stresses

KW - Transgenic rice

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M3 - Article

AN - SCOPUS:84858969214

SN - 0031-7454

VL - 94

SP - 29

EP - 37

JO - Philippine Agricultural Scientist

JF - Philippine Agricultural Scientist

IS - 1

ER -

Enhanced tolerance of transgenic rice overexpressing arabidopsis thaliana nucleoside diphosphate kinase (AtNDPK2) against various environmental stresses

Abstract

Keywords

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