Enhancement of γ-amminobutyric acid production by lactobacillus sakei B2-16 expressing glutamate decarboxylase from lactobacillus plantarum ATCC 14917

Moo Chang Kook; Myung Ji Seo; Chan Ick Cheigh; Sang Jae Lee; Yu Ryang Pyun; Hoon Park

doi:10.3839/jksabc.2010.123

Enhancement of γ-amminobutyric acid production by lactobacillus sakei B2-16 expressing glutamate decarboxylase from lactobacillus plantarum ATCC 14917

Moo Chang Kook, Myung Ji Seo, Chan Ick Cheigh, Sang Jae Lee, Yu Ryang Pyun, Hoon Park

Department of Food and Food Service Inudstry

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

Original language	English
Pages (from-to)	816-820
Number of pages	5
Journal	Journal of Applied Biological Chemistry
Volume	53
Issue number	6
DOIs	https://doi.org/10.3839/jksabc.2010.123
State	Published - 2010

Keywords

γ-amminobutyric acid
Glutamate decarboxylase
Lactobacillus plantarum
Lactobacillus sakei

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10.3839/jksabc.2010.123

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abstract = "To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.",

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T1 - Enhancement of γ-amminobutyric acid production by lactobacillus sakei B2-16 expressing glutamate decarboxylase from lactobacillus plantarum ATCC 14917

AU - Kook, Moo Chang

AU - Seo, Myung Ji

AU - Cheigh, Chan Ick

AU - Lee, Sang Jae

AU - Pyun, Yu Ryang

AU - Park, Hoon

PY - 2010

Y1 - 2010

N2 - To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

AB - To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

KW - γ-amminobutyric acid

KW - Glutamate decarboxylase

KW - Lactobacillus plantarum

KW - Lactobacillus sakei

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DO - 10.3839/jksabc.2010.123

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Enhancement of γ-amminobutyric acid production by lactobacillus sakei B2-16 expressing glutamate decarboxylase from lactobacillus plantarum ATCC 14917

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