Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7

Nyeon Sik Eum; Se Hyuk Yeom; Dae Hyuk Kwon; Hak Rin Kim; Shin Won Kang

doi:10.1016/j.snb.2009.09.054

Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7

Nyeon Sik Eum, Se Hyuk Yeom, Dae Hyuk Kwon, Hak Rin Kim, Shin Won Kang

School of Electronics Engineering

Research output: Contribution to journal › Review article › peer-review

66 Scopus citations

Abstract

In this study, GNRs (gold nanorods) were used to enhance the sensitivity of the biosensor for Escherichia coli O157:H7 in surface plasmon resonance (SPR) system. GNRs were designed and fabricated through seed-medicated growth and surface activation by alkanethiols for the attaching antibody. Antibodies for E. coli O157:H7 were immobilized on GNRs and measured by using UV-visible spectroscopy and sandwich assays were carried out to detect E. coli O157:H7 using Spreeta. This experiment is expected to play a key role in increasing sensitivity in other biosensor experiments and photothermal therapy.

Original language	English
Pages (from-to)	784-788
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2009.09.054
State	Published - 7 Jan 2010

Keywords

E. coli O157:H7
Gold nanorod
Nano-SPR
Spreeta
Surface plasmon resonance

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10.1016/j.snb.2009.09.054

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title = "Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7",

abstract = "In this study, GNRs (gold nanorods) were used to enhance the sensitivity of the biosensor for Escherichia coli O157:H7 in surface plasmon resonance (SPR) system. GNRs were designed and fabricated through seed-medicated growth and surface activation by alkanethiols for the attaching antibody. Antibodies for E. coli O157:H7 were immobilized on GNRs and measured by using UV-visible spectroscopy and sandwich assays were carried out to detect E. coli O157:H7 using Spreeta. This experiment is expected to play a key role in increasing sensitivity in other biosensor experiments and photothermal therapy.",

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T1 - Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7

AU - Eum, Nyeon Sik

AU - Yeom, Se Hyuk

AU - Kwon, Dae Hyuk

AU - Kim, Hak Rin

AU - Kang, Shin Won

PY - 2010/1/7

Y1 - 2010/1/7

N2 - In this study, GNRs (gold nanorods) were used to enhance the sensitivity of the biosensor for Escherichia coli O157:H7 in surface plasmon resonance (SPR) system. GNRs were designed and fabricated through seed-medicated growth and surface activation by alkanethiols for the attaching antibody. Antibodies for E. coli O157:H7 were immobilized on GNRs and measured by using UV-visible spectroscopy and sandwich assays were carried out to detect E. coli O157:H7 using Spreeta. This experiment is expected to play a key role in increasing sensitivity in other biosensor experiments and photothermal therapy.

AB - In this study, GNRs (gold nanorods) were used to enhance the sensitivity of the biosensor for Escherichia coli O157:H7 in surface plasmon resonance (SPR) system. GNRs were designed and fabricated through seed-medicated growth and surface activation by alkanethiols for the attaching antibody. Antibodies for E. coli O157:H7 were immobilized on GNRs and measured by using UV-visible spectroscopy and sandwich assays were carried out to detect E. coli O157:H7 using Spreeta. This experiment is expected to play a key role in increasing sensitivity in other biosensor experiments and photothermal therapy.

KW - E. coli O157:H7

KW - Gold nanorod

KW - Nano-SPR

KW - Spreeta

KW - Surface plasmon resonance

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DO - 10.1016/j.snb.2009.09.054

M3 - Review article

AN - SCOPUS:73149122923

SN - 0925-4005

VL - 143

SP - 784

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 2

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Enhancement of sensitivity using gold nanorods-Antibody conjugator for detection of E. coli O157:H7

Abstract

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