Ergosterol peroxides from the fruit body of Sparassis crispa

Yeong Geun Lee; Nhan Nguyen Thi; Hyoung Geun Kim; Dae Young Lee; Seung Eun Lee; Geum Soog Kim; Nam In Baek

doi:10.3839/jabc.2016.053

Ergosterol peroxides from the fruit body of Sparassis crispa

Yeong Geun Lee, Nhan Nguyen Thi, Hyoung Geun Kim, Dae Young Lee, Seung Eun Lee, Geum Soog Kim, Nam In Baek

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl SiO₂ and silica gel (SiO2) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as (3β,5α,8α,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-β-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.

Original language	English
Pages (from-to)	313-316
Number of pages	4
Journal	Journal of Applied Biological Chemistry
Volume	59
Issue number	4
DOIs	https://doi.org/10.3839/jabc.2016.053
State	Published - Dec 2016

Keywords

3-O-β-D-glucopyranosyl ergosterol peroxide
Ergosterol peroxide
Sparassis crispa
Sterol

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title = "Ergosterol peroxides from the fruit body of Sparassis crispa",

abstract = "Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl SiO2 and silica gel (SiO2) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as (3β,5α,8α,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-β-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.",

keywords = "3-O-β-D-glucopyranosyl ergosterol peroxide, Ergosterol peroxide, Sparassis crispa, Sterol",

author = "Lee, {Yeong Geun} and Thi, {Nhan Nguyen} and Kim, {Hyoung Geun} and Lee, {Dae Young} and Lee, {Seung Eun} and Kim, {Geum Soog} and Baek, {Nam In}",

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AU - Lee, Yeong Geun

AU - Thi, Nhan Nguyen

AU - Kim, Hyoung Geun

AU - Lee, Dae Young

AU - Lee, Seung Eun

AU - Kim, Geum Soog

AU - Baek, Nam In

N1 - Publisher Copyright: © The Korean Society for Applied Biological Chemistry 2016.

PY - 2016/12

Y1 - 2016/12

N2 - Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl SiO2 and silica gel (SiO2) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as (3β,5α,8α,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-β-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.

AB - Sparassis crispa fruits were extracted in 80 % MeOH, and the concentrated extract was partitioned into EtOAc, n-butyl alcohol, and water fractions. The repeated octadecyl SiO2 and silica gel (SiO2) column chromatographies for the EtOAc and nbutyl alcohol fractions led to isolation of two ergosterol peroxides. There chemical structures were determined as (3β,5α,8α,22E)-5,8-Epidioxyergosta-6,22-dien-3-ol (ergosterol peroxide) (1) and 3-O-β-D-glucopyranosyl ergosterol peroxide (2) based on spectroscopic data analyses including nuclear magnetic resonance, infrared spectrometry, and mass spectrometry (MS). Compounds 1 and 2 were for the first time isolated from S. crispa in this study.

KW - 3-O-β-D-glucopyranosyl ergosterol peroxide

KW - Ergosterol peroxide

KW - Sparassis crispa

KW - Sterol

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DO - 10.3839/jabc.2016.053

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SP - 313

EP - 316

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 4

ER -

Ergosterol peroxides from the fruit body of Sparassis crispa

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Keywords

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