Establishment and characterization of an immortalized human dermal papilla cell line

Seung Hyun Shin; Sang Yoon Park; Moon Kyu Kim; Jung Chul Kim; Young Kwan Sung

doi:10.5483/BMBRep.2011.44.8.512

Establishment and characterization of an immortalized human dermal papilla cell line

Seung Hyun Shin, Sang Yoon Park, Moon Kyu Kim, Jung Chul Kim, Young Kwan Sung

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40T-DPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/β-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.

Original language	English
Pages (from-to)	512-516
Number of pages	5
Journal	BMB Reports
Volume	44
Issue number	8
DOIs	https://doi.org/10.5483/BMBRep.2011.44.8.512
State	Published - Aug 2011

Keywords

Dermal papilla
Hair follicle
Immortalization
SV40T-transformation
hTERT

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title = "Establishment and characterization of an immortalized human dermal papilla cell line",

abstract = "Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40T-DPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/β-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.",

keywords = "Dermal papilla, Hair follicle, Immortalization, SV40T-transformation, hTERT",

author = "Shin, {Seung Hyun} and Park, {Sang Yoon} and Kim, {Moon Kyu} and Kim, {Jung Chul} and Sung, {Young Kwan}",

year = "2011",

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AU - Shin, Seung Hyun

AU - Park, Sang Yoon

AU - Kim, Moon Kyu

AU - Kim, Jung Chul

AU - Sung, Young Kwan

PY - 2011/8

Y1 - 2011/8

N2 - Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40T-DPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/β-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.

AB - Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40T-DPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/β-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.

KW - Dermal papilla

KW - Hair follicle

KW - Immortalization

KW - SV40T-transformation

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Establishment and characterization of an immortalized human dermal papilla cell line

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Keywords

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