Ethylene oxide suppresses boar sperm function during capacitation

Jae Hwan Jo, Claudine Uwamahoro, Seung Ik Jang, Eun Ju Jung, Woo Jin Lee, Jeong Won Bae, Dae Hyun Kim, Jun Koo Yi, Dong Yep Oh, Jae Jung Ha, Woo Sung Kwon

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Ethylene oxide (E.O) is an epoxide compound, and it has been utilized as a sterilizer or production of ether compounds in several industries. Although the toxic effects of E.O on bacteria and mammals have been reported, its effects on male reproductive toxicity during sperm capacitation are not fully understood. Therefore, this study was designed to evaluate the effects of E.O exposure during sperm capacitation. Boar spermatozoa were treated with various E.O concentrations (0, 0.1, 1, 10, and 100 μМ). After exposure, sperm motility, motion kinematics, capacitation status, intracellular ATP levels, cell viability, expression levels of protein kinase A (PKA) activation, and tyrosine phosphorylation were evaluated. Results revealed that E.O exposure significantly decreased sperm motility, motion kinematics, and intracellular ATP levels but significantly increased the capacitated spermatozoa. In addition, the PKA activation and tyrosine phosphorylation were abnormally changed. According to our results, E.O may cause toxic effects on sperm function during capacitation, which induces male reproductive toxicity. Consequently, we suggest that male reproductive toxicity should be considered when using E.O.

Original languageEnglish
Article number108678
JournalReproductive Toxicology
Volume129
DOIs
StatePublished - Oct 2024

Keywords

  • Capacitation
  • Ethylene oxide
  • Male reproductive toxicity
  • Sperm function

Fingerprint

Dive into the research topics of 'Ethylene oxide suppresses boar sperm function during capacitation'. Together they form a unique fingerprint.

Cite this