TY - JOUR
T1 - Ethylene oxide suppresses boar sperm function during capacitation
AU - Jo, Jae Hwan
AU - Uwamahoro, Claudine
AU - Jang, Seung Ik
AU - Jung, Eun Ju
AU - Lee, Woo Jin
AU - Bae, Jeong Won
AU - Kim, Dae Hyun
AU - Yi, Jun Koo
AU - Oh, Dong Yep
AU - Ha, Jae Jung
AU - Kwon, Woo Sung
N1 - Publisher Copyright:
© 2024 Elsevier Inc.
PY - 2024/10
Y1 - 2024/10
N2 - Ethylene oxide (E.O) is an epoxide compound, and it has been utilized as a sterilizer or production of ether compounds in several industries. Although the toxic effects of E.O on bacteria and mammals have been reported, its effects on male reproductive toxicity during sperm capacitation are not fully understood. Therefore, this study was designed to evaluate the effects of E.O exposure during sperm capacitation. Boar spermatozoa were treated with various E.O concentrations (0, 0.1, 1, 10, and 100 μМ). After exposure, sperm motility, motion kinematics, capacitation status, intracellular ATP levels, cell viability, expression levels of protein kinase A (PKA) activation, and tyrosine phosphorylation were evaluated. Results revealed that E.O exposure significantly decreased sperm motility, motion kinematics, and intracellular ATP levels but significantly increased the capacitated spermatozoa. In addition, the PKA activation and tyrosine phosphorylation were abnormally changed. According to our results, E.O may cause toxic effects on sperm function during capacitation, which induces male reproductive toxicity. Consequently, we suggest that male reproductive toxicity should be considered when using E.O.
AB - Ethylene oxide (E.O) is an epoxide compound, and it has been utilized as a sterilizer or production of ether compounds in several industries. Although the toxic effects of E.O on bacteria and mammals have been reported, its effects on male reproductive toxicity during sperm capacitation are not fully understood. Therefore, this study was designed to evaluate the effects of E.O exposure during sperm capacitation. Boar spermatozoa were treated with various E.O concentrations (0, 0.1, 1, 10, and 100 μМ). After exposure, sperm motility, motion kinematics, capacitation status, intracellular ATP levels, cell viability, expression levels of protein kinase A (PKA) activation, and tyrosine phosphorylation were evaluated. Results revealed that E.O exposure significantly decreased sperm motility, motion kinematics, and intracellular ATP levels but significantly increased the capacitated spermatozoa. In addition, the PKA activation and tyrosine phosphorylation were abnormally changed. According to our results, E.O may cause toxic effects on sperm function during capacitation, which induces male reproductive toxicity. Consequently, we suggest that male reproductive toxicity should be considered when using E.O.
KW - Capacitation
KW - Ethylene oxide
KW - Male reproductive toxicity
KW - Sperm function
UR - http://www.scopus.com/inward/record.url?scp=85199787297&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2024.108678
DO - 10.1016/j.reprotox.2024.108678
M3 - Article
C2 - 39068997
AN - SCOPUS:85199787297
SN - 0890-6238
VL - 129
JO - Reproductive Toxicology
JF - Reproductive Toxicology
M1 - 108678
ER -