Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Brandon W. Heimer; Tatyana A. Shatova; Jungkyu K. Lee; Kaja Kaastrup; Hadley D. Sikes

doi:10.1039/c4an00667d

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Brandon W. Heimer
, Tatyana A. Shatova
, Jungkyu K. Lee
, Kaja Kaastrup
, Hadley D. Sikes

Massachusetts Institute of Technology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

Original language	English
Pages (from-to)	3695-3701
Number of pages	7
Journal	The Analyst
Volume	139
Issue number	15
DOIs	https://doi.org/10.1039/c4an00667d
State	Published - 30 Jun 2014

Access to Document

10.1039/c4an00667d

Cite this

@article{631f3b3a1d2d49f992a6e5f984c76484,

title = "Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein",

abstract = "Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.",

author = "Heimer, \{Brandon W.\} and Shatova, \{Tatyana A.\} and Lee, \{Jungkyu K.\} and Kaja Kaastrup and Sikes, \{Hadley D.\}",

year = "2014",

month = jun,

day = "30",

doi = "10.1039/c4an00667d",

language = "English",

volume = "139",

pages = "3695--3701",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "15",

}

TY - JOUR

T1 - Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

AU - Heimer, Brandon W.

AU - Shatova, Tatyana A.

AU - Lee, Jungkyu K.

AU - Kaastrup, Kaja

AU - Sikes, Hadley D.

PY - 2014/6/30

Y1 - 2014/6/30

N2 - Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

AB - Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

UR - https://www.scopus.com/pages/publications/84903592255

U2 - 10.1039/c4an00667d

DO - 10.1039/c4an00667d

M3 - Article

C2 - 24824477

AN - SCOPUS:84903592255

SN - 0003-2654

VL - 139

SP - 3695

EP - 3701

JO - The Analyst

JF - The Analyst

IS - 15

ER -

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Abstract

Access to Document

Other files and links

Fingerprint

Cite this