Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Brandon W. Heimer; Tatyana A. Shatova; Jungkyu K. Lee; Kaja Kaastrup; Hadley D. Sikes

doi:10.1039/c4an00667d

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Brandon W. Heimer, Tatyana A. Shatova, Jungkyu K. Lee, Kaja Kaastrup, Hadley D. Sikes

Massachusetts Institute of Technology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

Original language	English
Pages (from-to)	3695-3701
Number of pages	7
Journal	The Analyst
Volume	139
Issue number	15
DOIs	https://doi.org/10.1039/c4an00667d
State	Published - 30 Jun 2014

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title = "Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein",

abstract = "Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.",

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AU - Heimer, Brandon W.

AU - Shatova, Tatyana A.

AU - Lee, Jungkyu K.

AU - Kaastrup, Kaja

AU - Sikes, Hadley D.

PY - 2014/6/30

Y1 - 2014/6/30

N2 - Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

AB - Hypermethylation of CpG islands in gene promoter regions has been shown to be a predictive biomarker for certain diseases. Most current methods for methylation profiling are not well-suited for clinical analysis. Here, we report the development of an inexpensive device and an epigenotyping assay with a format conducive to multiplexed analysis.

UR - https://www.scopus.com/pages/publications/84903592255

U2 - 10.1039/c4an00667d

DO - 10.1039/c4an00667d

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VL - 139

SP - 3695

EP - 3701

JO - The Analyst

JF - The Analyst

IS - 15

ER -

Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein

Abstract

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