Evaluation for biocompatibility of gentamicin-collagen sponge on the experimental animal wound model

The objective of this study was to compare the biocompatibility for local irritation and bioavailability of GentacolTM and Collatamp™, after single intramuscular loading in rats. Sixty-six male Sprague-Dawley rats were divided into 4 groups; (1) any test materials were not applied into the quadriceps muscles (control group, N = 6), (2) Gentamicin was injected into the quadriceps muscles (Gentamicin group, N = 6), (3) Collatamp was applied into the quadriceps muscles (Collatamp group, N = 27), and (4) Gentacol was applied into the quadriceps muscles (Gentacol group, N = 27). The concentration of gentamicin in muscles was gradually decreased with time after loaded in the both Gentacol™ and Collatamp™ loaded regions. No detectable gentamicin was observed in the plasma of Gentacol™ and Collatamp™ loaded rats. Histologically, moderate to severe inflammatory cell infiltrations including PMN, lymphoid cells and macrophages were observed with slight to moderate edematous changes of muscle fibers after intramuscular injection of gentamicin. However, these histopathological changes of gentamicin injection induced local irritations were dramatically decreases after intramuscular loading of Collatamp™ and Gentacol™. These results suggest Gentacol™ may show favorable local bioavailability and induce only slight local irritations as comparable as Collatamp™ without systemic exposures in the condition of this experiment.