TY - JOUR
T1 - Evaluation of kidney repair capacity using 99mTc-DMSA in ischemia/reperfusion injury models
AU - Kwak, Wonjung
AU - Jang, Hee Seong
AU - Belay, Takele
AU - Kim, Jinu
AU - Ha, Yeong Su
AU - Lee, Sang Woo
AU - Ahn, Byeong Cheol
AU - Lee, Jaetae
AU - Park, Kwon Moo
AU - Yoo, Jeongsoo
PY - 2011/3/4
Y1 - 2011/3/4
N2 - Quantitative 99mTc-DMSA renal uptake was studied in different renal ischemia/reperfusion (I/R) mice models for the assessment of renal repair capacity. Mice models of nephrectomy, uni- and bi-lateral I/R together with sham-operated mice were established. At 1h, 1d, 4d, 1, 2 and 3wk after I/R, 99mTc-DMSA (27.7±1.3MBq) was injected via tail vein and after 3h post-injection, the mice were scanned for 30min with pinhole equipped gamma camera. Higher uptake of 99mTc-DMSA was measured in normal kidneys of uni-lateral I/R model and nephrectomized kidney I/R model at 3wk post-surgery. Comparing the restoration capacities of the affected kidneys of nephrectomy, uni- and bi-lateral I/R models, higher repair capacity was observed in the nephrectomized model followed by bi-lateral then uni-lateral models. The normal kidney may retard the restoration of damaged kidney in uni-lateral I/R model. Moreover, 3wk after Uni-I/R, the size of injured kidney was significantly smaller than non-ischemic contralateral and sham operated kidneys, while nephrectomy I/R kidneys were significantly enlarged compared to all others at 3wk post-surgery. Very strong correlation between 99mTc-DMSA uptake and weight of dissected kidneys in I/R models was observed. Consistent with 99mTc-DMSA uptake results, all histological results indicate that kidney recovery after injury is correlated with the amount of intact tubules and kidney sizes. In summary, our study showed good potentials of 99mTc-DMSA scan as a promising non-invasive method for evaluation of kidney restoration after I/R injuries. Interestingly, mice with Bi-I/R injury showed faster repair capacity than those with uni-I/R.
AB - Quantitative 99mTc-DMSA renal uptake was studied in different renal ischemia/reperfusion (I/R) mice models for the assessment of renal repair capacity. Mice models of nephrectomy, uni- and bi-lateral I/R together with sham-operated mice were established. At 1h, 1d, 4d, 1, 2 and 3wk after I/R, 99mTc-DMSA (27.7±1.3MBq) was injected via tail vein and after 3h post-injection, the mice were scanned for 30min with pinhole equipped gamma camera. Higher uptake of 99mTc-DMSA was measured in normal kidneys of uni-lateral I/R model and nephrectomized kidney I/R model at 3wk post-surgery. Comparing the restoration capacities of the affected kidneys of nephrectomy, uni- and bi-lateral I/R models, higher repair capacity was observed in the nephrectomized model followed by bi-lateral then uni-lateral models. The normal kidney may retard the restoration of damaged kidney in uni-lateral I/R model. Moreover, 3wk after Uni-I/R, the size of injured kidney was significantly smaller than non-ischemic contralateral and sham operated kidneys, while nephrectomy I/R kidneys were significantly enlarged compared to all others at 3wk post-surgery. Very strong correlation between 99mTc-DMSA uptake and weight of dissected kidneys in I/R models was observed. Consistent with 99mTc-DMSA uptake results, all histological results indicate that kidney recovery after injury is correlated with the amount of intact tubules and kidney sizes. In summary, our study showed good potentials of 99mTc-DMSA scan as a promising non-invasive method for evaluation of kidney restoration after I/R injuries. Interestingly, mice with Bi-I/R injury showed faster repair capacity than those with uni-I/R.
KW - Tc-DMSA
KW - Imaging
KW - Kidney repair
KW - Renal ischemia/reperfusion
UR - http://www.scopus.com/inward/record.url?scp=79952187947&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2011.01.085
DO - 10.1016/j.bbrc.2011.01.085
M3 - Article
C2 - 21277288
AN - SCOPUS:79952187947
SN - 0006-291X
VL - 406
SP - 7
EP - 12
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -