Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[124I]iodobenzoate for cell tracking

Min Hwan Kim; Kyo Chul Lee; Gwang Il An; Sang Keun Woo; Noh Won Park; Byung Il Kim; Ki Dong Eom; Kwang Il Kim; Tae Sup Lee; Chan Wha Kim; Jeongsoo Yoo; Joo Hyun Kang; Yong Jin Lee

doi:10.1016/j.apradiso.2015.12.039

Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking

Min Hwan Kim, Kyo Chul Lee, Gwang Il An, Sang Keun Woo, Noh Won Park, Byung Il Kim, Ki Dong Eom, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Jeongsoo Yoo, Joo Hyun Kang, Yong Jin Lee

Department of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

This study was aimed to evaluate the effect of ¹²⁴I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [¹²⁴I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [¹²⁴I]HIB-labeled ADSCs. We demonstrated that ¹²⁴I-labeling with HIB did not affect the biological properties of ADSCs.

Original language	English
Pages (from-to)	116-123
Number of pages	8
Journal	Applied Radiation and Isotopes
Volume	108
DOIs	https://doi.org/10.1016/j.apradiso.2015.12.039
State	Published - 1 Feb 2016

Keywords

Adipose-derived stem cells
Direct labeling agent
Hexadecyl-4-[I]iodobenzoate
Microarray
Myocardial infarction

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10.1016/j.apradiso.2015.12.039

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Kim, M. H., Lee, K. C., An, G. I., Woo, S. K., Park, N. W., Kim, B. I., Eom, K. D., Kim, K. I., Lee, T. S., Kim, C. W., Yoo, J., Kang, J. H., & Lee, Y. J. (2016). Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking. Applied Radiation and Isotopes, 108, 116-123. https://doi.org/10.1016/j.apradiso.2015.12.039

@article{18092a9b3ba14b5dbf1a74400109fe2d,

title = "Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[124I]iodobenzoate for cell tracking",

abstract = "This study was aimed to evaluate the effect of 124I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [124I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [124I]HIB-labeled ADSCs. We demonstrated that 124I-labeling with HIB did not affect the biological properties of ADSCs.",

keywords = "Adipose-derived stem cells, Direct labeling agent, Hexadecyl-4-[I]iodobenzoate, Microarray, Myocardial infarction",

author = "Kim, {Min Hwan} and Lee, {Kyo Chul} and An, {Gwang Il} and Woo, {Sang Keun} and Park, {Noh Won} and Kim, {Byung Il} and Eom, {Ki Dong} and Kim, {Kwang Il} and Lee, {Tae Sup} and Kim, {Chan Wha} and Jeongsoo Yoo and Kang, {Joo Hyun} and Lee, {Yong Jin}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2016",

month = feb,

day = "1",

doi = "10.1016/j.apradiso.2015.12.039",

language = "English",

volume = "108",

pages = "116--123",

journal = "Applied Radiation and Isotopes",

issn = "0969-8043",

publisher = "Elsevier Ltd.",

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Kim, MH, Lee, KC, An, GI, Woo, SK, Park, NW, Kim, BI, Eom, KD, Kim, KI, Lee, TS, Kim, CW, Yoo, J, Kang, JH & Lee, YJ 2016, 'Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking', Applied Radiation and Isotopes, vol. 108, pp. 116-123. https://doi.org/10.1016/j.apradiso.2015.12.039

TY - JOUR

T1 - Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[124I]iodobenzoate for cell tracking

AU - Kim, Min Hwan

AU - Lee, Kyo Chul

AU - An, Gwang Il

AU - Woo, Sang Keun

AU - Park, Noh Won

AU - Kim, Byung Il

AU - Eom, Ki Dong

AU - Kim, Kwang Il

AU - Lee, Tae Sup

AU - Kim, Chan Wha

AU - Yoo, Jeongsoo

AU - Kang, Joo Hyun

AU - Lee, Yong Jin

PY - 2016/2/1

Y1 - 2016/2/1

N2 - This study was aimed to evaluate the effect of 124I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [124I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [124I]HIB-labeled ADSCs. We demonstrated that 124I-labeling with HIB did not affect the biological properties of ADSCs.

AB - This study was aimed to evaluate the effect of 124I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [124I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [124I]HIB-labeled ADSCs. We demonstrated that 124I-labeling with HIB did not affect the biological properties of ADSCs.

KW - Adipose-derived stem cells

KW - Direct labeling agent

KW - Hexadecyl-4-[I]iodobenzoate

KW - Microarray

KW - Myocardial infarction

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SN - 0969-8043

VL - 108

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JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

ER -

Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[¹²⁴I]iodobenzoate for cell tracking

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