Evaluation of the cell depleted layer of blood flow in vitro using confocal microscopic PIV method

S. H. Lim, H. Lee, C. Y. Lee, C. W. Park

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In the present study, we employed the laser scanning confocal microscope to image entire blood flow with accurate red blood cell imaging of 0.001 mm spatial resolution. In vitro blood flow of rat with different hematocrit ratios was simulated inside a 100and 300-micron opaque tube. The scanning rate of confocal microscope was 30 fps with 500 x 500 pixels of image. As a result, we can obtain clear images of RBCs to which is enough to be used as tracer particle directly to get the velocity vector field of blood flow by performing particle image velocimetry (PIV) technique non-invasively. Based on the present novel optical application, we can easily indicate the presence of cell depleted layer of blood flow in vitro and its boundaries.

Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing X
Subtitle of host publicationToward Point-of-Care Diagnostics
DOIs
StatePublished - 2010
EventOptical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics - San Francisco, CA, United States
Duration: 25 Jan 201026 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7572
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics
Country/TerritoryUnited States
CitySan Francisco, CA
Period25/01/1026/01/10

Keywords

  • Blood flow
  • Cell depleted layer
  • Confocal microscopy
  • Particle image velocimetry

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