Exclusively NOESY-based automated NMR assignment and structure determination of proteins

Teppei Ikeya; Jun Goo Jee; Yoshiki Shigemitsu; Junpei Hamatsu; Masaki Mishima; Yutaka Ito; Masatsune Kainosho; Peter Güntert

doi:10.1007/s10858-011-9502-8

Exclusively NOESY-based automated NMR assignment and structure determination of proteins

Teppei Ikeya, Jun Goo Jee, Yoshiki Shigemitsu, Junpei Hamatsu, Masaki Mishima, Yutaka Ito, Masatsune Kainosho, Peter Güntert

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

Original language	English
Pages (from-to)	137-146
Number of pages	10
Journal	Journal of Biomolecular NMR
Volume	50
Issue number	2
DOIs	https://doi.org/10.1007/s10858-011-9502-8
State	Published - Jun 2011

Keywords

Automated assignment
CYANA
FLYA
Protein structures
SAIL

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10.1007/s10858-011-9502-8

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abstract = "A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.",

keywords = "Automated assignment, CYANA, FLYA, Protein structures, SAIL",

author = "Teppei Ikeya and Jee, {Jun Goo} and Yoshiki Shigemitsu and Junpei Hamatsu and Masaki Mishima and Yutaka Ito and Masatsune Kainosho and Peter G{\"u}ntert",

year = "2011",

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T1 - Exclusively NOESY-based automated NMR assignment and structure determination of proteins

AU - Ikeya, Teppei

AU - Jee, Jun Goo

AU - Shigemitsu, Yoshiki

AU - Hamatsu, Junpei

AU - Mishima, Masaki

AU - Ito, Yutaka

AU - Kainosho, Masatsune

AU - Güntert, Peter

PY - 2011/6

Y1 - 2011/6

N2 - A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

AB - A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.

KW - Automated assignment

KW - CYANA

KW - FLYA

KW - Protein structures

KW - SAIL

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U2 - 10.1007/s10858-011-9502-8

DO - 10.1007/s10858-011-9502-8

M3 - Article

C2 - 21448734

AN - SCOPUS:80051691505

SN - 0925-2738

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EP - 146

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 2

ER -

Exclusively NOESY-based automated NMR assignment and structure determination of proteins

Abstract

Keywords

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