TY - JOUR
T1 - Expression of a functional human Type I interferon receptor in hamster cells
T2 - Application of functional yeast artificial chromosome (YAC) screening
AU - Soh, Jaemog
AU - Mariano, Thomas M.
AU - Lim, Jin Kyu
AU - Izotova, Lara
AU - Mirochnitchenko, Olga
AU - Schwartz, Barbara
AU - Langer, Jerome A.
AU - Pestka, Sidney
PY - 1994/7/8
Y1 - 1994/7/8
N2 - The previously cloned human interferon α/β (Hu-IFN-α/β; Type I interferon) receptor cDNA appears to be only one component of a receptor complex since expression of the cDNA in mouse cells confers sensitivity only to Hu-IFN-αB2, but a monoclonal antibody against this cloned receptor subunit inhibits biological activities of Hu-IFN-αA, Hu-IFN-αB2, Hu-IFN- ω, and Hu-IFN-β. Here we report that a yeast artificial chromosome (YAC) containing a segment of human chromosome 21 introduced into Chinese hamster ovary (CHO) cells confers upon these cells a greatly enhanced response to Hu- IFN-αA and Hu-IFN-αB2 as well as an increased response to Hu-IFN-ω, Hu- IFN-αA/D (Bgl), and Hu-IFN-β. These responses were measured by induction of class I MHC antigens and by protection against encephalomyocarditis virus and vesicular stomatitis virus. Furthermore, these cells exhibit specific high affinity binding of Hu-IFN-αA and Hu-IFN-αB2, Hu-IFN-β, and Hu-IFN-ω. The results indicate that all the genes necessary to reconstitute a biologically active Type I human IFN receptor complex are located within the human DNA insert of this YAC clone.
AB - The previously cloned human interferon α/β (Hu-IFN-α/β; Type I interferon) receptor cDNA appears to be only one component of a receptor complex since expression of the cDNA in mouse cells confers sensitivity only to Hu-IFN-αB2, but a monoclonal antibody against this cloned receptor subunit inhibits biological activities of Hu-IFN-αA, Hu-IFN-αB2, Hu-IFN- ω, and Hu-IFN-β. Here we report that a yeast artificial chromosome (YAC) containing a segment of human chromosome 21 introduced into Chinese hamster ovary (CHO) cells confers upon these cells a greatly enhanced response to Hu- IFN-αA and Hu-IFN-αB2 as well as an increased response to Hu-IFN-ω, Hu- IFN-αA/D (Bgl), and Hu-IFN-β. These responses were measured by induction of class I MHC antigens and by protection against encephalomyocarditis virus and vesicular stomatitis virus. Furthermore, these cells exhibit specific high affinity binding of Hu-IFN-αA and Hu-IFN-αB2, Hu-IFN-β, and Hu-IFN-ω. The results indicate that all the genes necessary to reconstitute a biologically active Type I human IFN receptor complex are located within the human DNA insert of this YAC clone.
UR - http://www.scopus.com/inward/record.url?scp=0028361818&partnerID=8YFLogxK
M3 - Article
C2 - 8027072
AN - SCOPUS:0028361818
SN - 0021-9258
VL - 269
SP - 18102
EP - 18110
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 27
ER -