Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese

Chang Kil Kim; Jeung Sul Han; Hyun Suk Lee; Joung Youl Oh; Thoshiro Shigaki; Sung Hun Park; Kendal Hirschi

doi:10.1007/s00299-006-0214-6

Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese

Chang Kil Kim, Jeung Sul Han, Hyun Suk Lee, Joung Youl Oh, Thoshiro Shigaki, Sung Hun Park, Kendal Hirschi

Department of Horticultural Science

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the "B" domain from CAX1] that has enhanced calcium (Ca²⁺) substrate specificity and lost the manganese (Mn²⁺) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50-65% more calcium (Ca²⁺) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn ²⁺). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca²⁺ nutrient enrichment of potatoes with reduced accumulation of undesirable metals.

Original language	English
Pages (from-to)	1226-1232
Number of pages	7
Journal	Plant Cell Reports
Volume	25
Issue number	11
DOIs	https://doi.org/10.1007/s00299-006-0214-6
State	Published - Nov 2006

Keywords

Arabidopsis
Calcium
H/Ca antiporter
Manganese
Potato

Access to Document

10.1007/s00299-006-0214-6

Cite this

@article{46c1eac1cd2e46cfa8c38a464c21ab47,

title = "Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese",

abstract = "Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the {"}B{"} domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50-65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn 2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals.",

keywords = "Arabidopsis, Calcium, H/Ca antiporter, Manganese, Potato",

author = "Kim, {Chang Kil} and Han, {Jeung Sul} and Lee, {Hyun Suk} and Oh, {Joung Youl} and Thoshiro Shigaki and Park, {Sung Hun} and Kendal Hirschi",

year = "2006",

month = nov,

doi = "10.1007/s00299-006-0214-6",

language = "English",

volume = "25",

pages = "1226--1232",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "11",

}

TY - JOUR

T1 - Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese

AU - Kim, Chang Kil

AU - Han, Jeung Sul

AU - Lee, Hyun Suk

AU - Oh, Joung Youl

AU - Shigaki, Thoshiro

AU - Park, Sung Hun

AU - Hirschi, Kendal

PY - 2006/11

Y1 - 2006/11

N2 - Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the "B" domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50-65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn 2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals.

AB - Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the "B" domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50-65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn 2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals.

KW - Arabidopsis

KW - Calcium

KW - H/Ca antiporter

KW - Manganese

KW - Potato

UR - http://www.scopus.com/inward/record.url?scp=33750289876&partnerID=8YFLogxK

U2 - 10.1007/s00299-006-0214-6

DO - 10.1007/s00299-006-0214-6

M3 - Article

C2 - 17024452

AN - SCOPUS:33750289876

SN - 0721-7714

VL - 25

SP - 1226

EP - 1232

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 11

ER -

Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this