TY - JOUR
T1 - Expression of sphingosine-1-phosphate receptor 1 in neuroinflammation of canine brains
AU - Yun, Taesik
AU - Kim, Sanggu
AU - Koo, Yoonhoi
AU - Chae, Yeon
AU - Lee, Dohee
AU - Kim, Hakhyun
AU - Yang, Mhan Pyo
AU - Kang, Byeong Teck
AU - Kim, Soochong
N1 - Publisher Copyright:
© 2024
PY - 2024/5/1
Y1 - 2024/5/1
N2 - Sphingosine-1-phosphate (S1P) is a signaling lipid mediator that is involved in multiple biological processes. The S1P/S1P receptor (S1PR) signaling pathway has an important role in the central nervous system. It contributes to physiologic cellular homeostasis and is also associated with neuroinflammation. Therefore, this study was performed to evaluate the expression of S1PR in dogs with meningoencephalitis of unknown etiology (MUE) and experimental autoimmune encephalomyelitis (EAE). The analysis used 12 brain samples from three neurologically normal dogs, seven dogs with MUE, and two canine EAE models. Anti-S1PR1 antibody was used for immunohistochemistry. In normal brain tissues, S1PR1s were expressed on neurons, astrocytes, oligodendrocytes, and endothelial cells. In MUE and EAE lesions, there was positive staining of S1PR1 on leukocytes. Furthermore, the expression of S1PR1 on neurons, astrocytes, oligodendrocytes, and endothelial cells was upregulated compared to normal brains. This study shows that S1PR1s are expressed in normal brain tissues and leukocytes in inflammatory lesions, and demonstrates the upregulation of S1PR1 expression on nervous system cells in inflammatory lesions of MUE and EAE. These findings indicate that S1P/S1PR signaling pathway might involve physiologic homeostasis and neuroinflammation and represent potential targets for S1PR modulators to treat MUE.
AB - Sphingosine-1-phosphate (S1P) is a signaling lipid mediator that is involved in multiple biological processes. The S1P/S1P receptor (S1PR) signaling pathway has an important role in the central nervous system. It contributes to physiologic cellular homeostasis and is also associated with neuroinflammation. Therefore, this study was performed to evaluate the expression of S1PR in dogs with meningoencephalitis of unknown etiology (MUE) and experimental autoimmune encephalomyelitis (EAE). The analysis used 12 brain samples from three neurologically normal dogs, seven dogs with MUE, and two canine EAE models. Anti-S1PR1 antibody was used for immunohistochemistry. In normal brain tissues, S1PR1s were expressed on neurons, astrocytes, oligodendrocytes, and endothelial cells. In MUE and EAE lesions, there was positive staining of S1PR1 on leukocytes. Furthermore, the expression of S1PR1 on neurons, astrocytes, oligodendrocytes, and endothelial cells was upregulated compared to normal brains. This study shows that S1PR1s are expressed in normal brain tissues and leukocytes in inflammatory lesions, and demonstrates the upregulation of S1PR1 expression on nervous system cells in inflammatory lesions of MUE and EAE. These findings indicate that S1P/S1PR signaling pathway might involve physiologic homeostasis and neuroinflammation and represent potential targets for S1PR modulators to treat MUE.
KW - Dog
KW - EAE
KW - Experimental autoimmune encephalomyelitis
KW - MUE
KW - Meningoencephalitis of unknown etiology
KW - S1PR1
UR - http://www.scopus.com/inward/record.url?scp=85182378234&partnerID=8YFLogxK
U2 - 10.1016/j.tcam.2024.100847
DO - 10.1016/j.tcam.2024.100847
M3 - Article
C2 - 38182045
AN - SCOPUS:85182378234
SN - 1938-9736
VL - 60
JO - Topics in Companion Animal Medicine
JF - Topics in Companion Animal Medicine
M1 - 100847
ER -