Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing

Gyeong In Mun; Soojin Park; Joachim Kremerskothen; Yong Chool Boo

doi:10.1016/j.febslet.2014.02.012

Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing

Gyeong In Mun, Soojin Park, Joachim Kremerskothen, Yong Chool Boo

Department of Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.

Original language	English
Pages (from-to)	1024-1030
Number of pages	7
Journal	FEBS Letters
Volume	588
Issue number	6
DOIs	https://doi.org/10.1016/j.febslet.2014.02.012
State	Published - 18 Mar 2014

Keywords

Endothelial cell
Shear stress
Synaptopodin
Wound healing

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10.1016/j.febslet.2014.02.012

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title = "Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing",

abstract = "We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.",

keywords = "Endothelial cell, Shear stress, Synaptopodin, Wound healing",

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T1 - Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing

AU - Mun, Gyeong In

AU - Park, Soojin

AU - Kremerskothen, Joachim

AU - Boo, Yong Chool

PY - 2014/3/18

Y1 - 2014/3/18

N2 - We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.

AB - We examined the hypothesis that certain actin binding proteins might be upregulated by laminar shear stress (LSS) and could contribute to endothelial wound healing. Analysis of mRNA expression profiles of human umbilical vein endothelial cells under static and LSS-exposed conditions provided a list of LSS-induced actin binding proteins including synaptopodin (SYNPO) whose endothelial expression has not been previously reported. Additional studies demonstrated that SYNPO is a key mediator of endothelial wound healing because small interfering RNA-mediated suppression of SYNPO attenuated wound closure under LSS whereas overexpression of exogenous SYNPO enhanced endothelial wound closure in the absence of LSS. This study suggests that LSS-induced actin binding proteins including SYNPO may play a critical role in the endothelial wound healing stimulated by LSS.

KW - Endothelial cell

KW - Shear stress

KW - Synaptopodin

KW - Wound healing

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DO - 10.1016/j.febslet.2014.02.012

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Expression of synaptopodin in endothelial cells exposed to laminar shear stress and its role in endothelial wound healing

Abstract

Keywords

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