Expression pattern of low-temperature-related genes by the treatment of AFP in the cryopreservation of potato shoot tips

Ji Hyang Seo, Su Min Jeon, Aung Htay Naing, Jeung Sul Han, In Hwan Park, Chang Kil Kim

Research output: Contribution to journalArticlepeer-review

Abstract

The expression profiles of low-temperature-related genes were examined in in vitro potatoes exposed to a cold condition for 1-3 days. The expression levels of PPI1 and CI21B genes were lineraly elevated from day 1 to day 3, while the opposite trend was observed for CBF4 and CI21A. In addition, the expression of the genes CI21A and CI21B varied, along with specific tissues (leaf, stem, and tuber) and the treatment periods. Therefore, potato shoot tips were cryopreserved with LS and PVS2 containing different oncentrations of AFP. It can thus be inferred that the presence of AFP in LS and PVS2 was likely to elevate expression pattern of the genes. Furthermore, the concentration of AFP (1,500 ng/ml for LS and 500 ng/mg for PVS2) was the best for the cryopreservation of potatoes.

Original languageEnglish
Pages (from-to)478-483
Number of pages6
JournalJournal of Plant Biotechnology
Volume44
Issue number4
DOIs
StatePublished - 31 Dec 2017

Keywords

  • LS
  • PVS2
  • Real-time PCR
  • Vitrification

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