Expression patterns of germ cell-specific phosducin-like 2 during testicular and ovarian development in chickens

Ying Hui Zheng, Deivendran Rengaraj, Kyung Je Park, Sang In Lee, Jae Yong Han

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Phosducin (PDC) is a photoreceptor cell-specific protein that is phosphorylated by cyclic nucleotide-dependent protein kinase. PDC and PDC-like proteins (PDCL, PDCL2, and PDCL3) are members of a conserved family of small thioredoxin-like proteins that modulate the β- and γ-subunits of G-proteins. In mammals, Pdc, Pdcl, and Pdc3 genes show ubiquitous expression; however, Pdcl2 gene expression is limited to the testis and ovary. The aim of the present study was to examine the expression patterns of chicken Pdcl2 (cPdcl2) during testicular and ovarian development. Protein sequence comparisons performed using the CLUSTAL X program revealed that the amino acid sequences and potential phosphorylation sites of cPDCL2 and mammalian PDCL2 proteins were highly conserved. Quantitative real-time PCR analysis revealed that cPdcl2 was differentially expressed in the testis and ovary. Specifically, cPdcl2 expression was detected at low levels in the ovary at all time points. In the testis, cPdcl2 expression was detected at low levels until 5 weeks of age. At 8 weeks of age, however, zPdcU showed increased expression levels in the testis. Using in situ hybridization, we detected high levels of cPdcl2 expression in the testis, particularly in the spermatocytes and round spermatids. In summary, our data describe expression patterns of germ cell-specific Pdcl2 during testicular and ovarian development in chickens.

Original languageEnglish
Pages (from-to)1000-1006
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume23
Issue number8
DOIs
StatePublished - Aug 2010

Keywords

  • Chickens
  • In situ Hybridization
  • Pdc12
  • qRT-PCR

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