TY - JOUR
T1 - Expression, purification, crystallization and initial crystallographic characterization of the p-hydroxybenzoate hydroxylase from Corynebacterium glutamicum
AU - Kwon, Soo Young
AU - Kang, Beom Sik
AU - Kim, Ghyung Hwa
AU - Kim, Kyung Jin
PY - 2007
Y1 - 2007
N2 - p-Hydroxybenzoate hydroxylase (PHBH) is an FAD-dependent mono-oxygenase that catalyzes the hydroxylation of p-hydroxybenzoate (pOHB) to 3,4-dihydroxybenzoate in an NADPH-dependent reaction and plays an important role in the biodegradation of aromatic compounds. PHBH from Corynebacterium glutamicum was crystallized using the hanging-drop vapour-diffusion method in the presence of NaH2PO4 and K2HPO4 as precipitants. X-ray diffraction data were collected to a maximum resolution of 2.5 Å on a synchrotron beamline. The crystal belongs to the hexagonal space group P6322, with unit-cell parameters a = b = 94.72, c = 359.68 Å, γ = 120°. The asymmetric unit contains two molecules, corresponding to a packing density of 2.65 Å3 Da-1. The structure was solved by molecular replacement. Structure refinement is in progress.
AB - p-Hydroxybenzoate hydroxylase (PHBH) is an FAD-dependent mono-oxygenase that catalyzes the hydroxylation of p-hydroxybenzoate (pOHB) to 3,4-dihydroxybenzoate in an NADPH-dependent reaction and plays an important role in the biodegradation of aromatic compounds. PHBH from Corynebacterium glutamicum was crystallized using the hanging-drop vapour-diffusion method in the presence of NaH2PO4 and K2HPO4 as precipitants. X-ray diffraction data were collected to a maximum resolution of 2.5 Å on a synchrotron beamline. The crystal belongs to the hexagonal space group P6322, with unit-cell parameters a = b = 94.72, c = 359.68 Å, γ = 120°. The asymmetric unit contains two molecules, corresponding to a packing density of 2.65 Å3 Da-1. The structure was solved by molecular replacement. Structure refinement is in progress.
KW - Corynebacterium glutamicum
KW - FAD-dependent monooxygenases
KW - p-hydroxybenzoate hydroxylase
UR - http://www.scopus.com/inward/record.url?scp=36248968090&partnerID=8YFLogxK
U2 - 10.1107/S1744309107046386
DO - 10.1107/S1744309107046386
M3 - Article
C2 - 18007046
AN - SCOPUS:36248968090
SN - 1744-3091
VL - 63
SP - 944
EP - 946
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -