Extracelluar signal-regulated kinase-dependent nitric oxide production from macrophage-like cells by lactic acid bacteria

Se Eun Byeon, Dae Sung Yoo, Jaehwi Lee, Suk Kim, Man Hee Rhee, Hwa Jin Park, Jae Youl Cho

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Lactic acid bacteria (LAB) are considered as probiotics with immunostimulatory property. In this study, we investigated the molecular mechanism of its immunostimulating potency on macrophages using combined preparation of LAB (cpLAB). cpLAB is able to strongly stimulate nitric oxide (NO) production as well as inducible NO synthase (iNOS) expression from macrophage-like RAW264.7 cells. The cpLAB-induced NO release seemed to be mediated by extracellular signal-regulated kinase (ERK) but not p38 and C-Jun N-terminal kinase (JNK), since U0126, an ERK inhibitor, clearly suppressed NO production. cpLAB significantly diminished the binding of toll like receptor (TLR)-2 antibody up to 25%, implying that cpLAB-mediated activation of macrophages may be required for the functional activation of TLR-2, but not TLR-4. Therefore, our data suggest that cpLAB may directly allow macrophages to immunostimulating potency via activation of TLR-2 and ERK.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
JournalBiomolecules and Therapeutics
Volume17
Issue number3
DOIs
StatePublished - Jul 2009

Keywords

  • Extracelluar signal-regulated kinase
  • Lactic acid bacteria
  • Nitric oxide

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