Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Jun Kyu Kim; Young Jin Youn; Yu Bin Lee; Sun Hwa Kim; Dong Keun Song; Minsang Shin; Hee Kyung Jin; Jae sung Bae; Sanjeeb Shrestha; Chang Won Hong

doi:10.1038/s41598-021-87891-8

Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Jun Kyu Kim, Young Jin Youn, Yu Bin Lee, Sun Hwa Kim, Dong Keun Song, Minsang Shin, Hee Kyung Jin, Jae sung Bae, Sanjeeb Shrestha, Chang Won Hong

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.

Original language	English
Article number	8289
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-87891-8
State	Published - Dec 2021

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title = "Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics",

abstract = "Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.",

author = "Kim, {Jun Kyu} and Youn, {Young Jin} and Lee, {Yu Bin} and Kim, {Sun Hwa} and Song, {Dong Keun} and Minsang Shin and Jin, {Hee Kyung} and Bae, {Jae sung} and Sanjeeb Shrestha and Hong, {Chang Won}",

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AU - Kim, Jun Kyu

AU - Youn, Young Jin

AU - Lee, Yu Bin

AU - Kim, Sun Hwa

AU - Song, Dong Keun

AU - Shin, Minsang

AU - Jin, Hee Kyung

AU - Bae, Jae sung

AU - Shrestha, Sanjeeb

AU - Hong, Chang Won

PY - 2021/12

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AB - Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.

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Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

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