Fabrication of an ultrafine fish gelatin nanofibrous web from an aqueous solution by electrospinning

Hyo Won Kwak, Munju Shin, Jeong Yun Lee, Haesung Yun, Dae Woong Song, Yesol Yang, Bong Seob Shin, Young Hwan Park, Ki Hoon Lee

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Electrospinning of aqueous gelatin solution obtained from bovine or porcine sources has been difficult to achieve without additional facilities, such as a temperature control oven or heating cover. Gelatin from cold-water fish has low contents of proline (Pro) and hydroxyproline (Hyp) compared with mammalian-derived gelatin. For this reason, the fish-derived gelatin maintains a sol state without showing gelation behavior at room temperature. In the present study, we prepared an ultrafine fish gelatin nanofibrous web by electrospinning from aqueous solutions without any additive polymers or temperature control facilities. The concentration and viscosity of fish gelatin are the most important factor in determining the electrospinnability and fiber diameter. Electrospinning of aqueous fish gelatin has the highest nanofiber productivity compared to other organic solvent systems. Using glutaraldehyde vapor (GTA), the water stability was improved and substantial enhancement was achieved in the mechanical properties. Finally, the cytotoxicity of a fish gelatin nanofibrous scaffold was evaluated based on a cell proliferation study by culturing human dermal fibroblasts (HDFs) compared with a fish gelatin film and nanofibrous mat from mammalian gelatin. The result shows better initial cell attachment and proliferation compared with the fish gelatin film and no significant difference compared with mammalian-derived gelatin nanofibrous mat. We expect that electrospinning of aqueous fish gelatin could be an effective alternative mammalian gelatin source.

Original languageEnglish
Pages (from-to)1092-1103
Number of pages12
JournalInternational Journal of Biological Macromolecules
Volume102
DOIs
StatePublished - 1 Sep 2017

Keywords

  • Concentration
  • Electrospinning
  • Fish gelatin
  • Nanofiber
  • Viscosity

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