Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor

Palanisamy Gomathi; Dhanusuraman Ragupathy; Jin Hyun Choi; Jeong Hyun Yeum; Soo Chool Lee; Jae Chang Kim; Sang Hak Lee; Han Do Ghim

doi:10.1016/j.snb.2010.10.005

Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor

Palanisamy Gomathi, Dhanusuraman Ragupathy, Jin Hyun Choi, Jeong Hyun Yeum, Soo Chool Lee, Jae Chang Kim, Sang Hak Lee, Han Do Ghim

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of ∼50-100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF-AuNPs/ChOx biosensor. The CSNF-AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1-45 μM), good sensitivity (1.02 μA/μM), low response time (∼5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol.

Original language	English
Pages (from-to)	44-49
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	153
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2010.10.005
State	Published - 31 Mar 2011

Keywords

Biosensor
Chitosan
Cholesterol oxidase
Gold nanoparticles

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title = "Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor",

abstract = "A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of ∼50-100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF-AuNPs/ChOx biosensor. The CSNF-AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1-45 μM), good sensitivity (1.02 μA/μM), low response time (∼5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol.",

keywords = "Biosensor, Chitosan, Cholesterol oxidase, Gold nanoparticles",

author = "Palanisamy Gomathi and Dhanusuraman Ragupathy and Choi, {Jin Hyun} and Yeum, {Jeong Hyun} and Lee, {Soo Chool} and Kim, {Jae Chang} and Lee, {Sang Hak} and Ghim, {Han Do}",

year = "2011",

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doi = "10.1016/j.snb.2010.10.005",

language = "English",

volume = "153",

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AU - Gomathi, Palanisamy

AU - Ragupathy, Dhanusuraman

AU - Choi, Jin Hyun

AU - Yeum, Jeong Hyun

AU - Lee, Soo Chool

AU - Kim, Jae Chang

AU - Lee, Sang Hak

AU - Ghim, Han Do

PY - 2011/3/31

Y1 - 2011/3/31

N2 - A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of ∼50-100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF-AuNPs/ChOx biosensor. The CSNF-AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1-45 μM), good sensitivity (1.02 μA/μM), low response time (∼5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol.

AB - A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of ∼50-100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF-AuNPs/ChOx biosensor. The CSNF-AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1-45 μM), good sensitivity (1.02 μA/μM), low response time (∼5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol.

KW - Biosensor

KW - Chitosan

KW - Cholesterol oxidase

KW - Gold nanoparticles

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IS - 1

ER -

Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor

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Keywords

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