Fabrications of a continuous-flow PCR-chip using dry film resist

  • D. Y. Kong
  • , S. W. Kim
  • , C. T. Seo
  • , J. H. Lee
  • , C. S. Cho
  • , B. H. Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

In most miniaturized PCR-chip, the sample solution is introduced into a small chamber, and the DNA amplification is performed by thermal cycling. However, chamber heating and cooling rates are relatively low. But, the continuous-flow type PCR-chip involves the transfer of a PCR mixture through a micro-fluidic channel which passes through three different temperature regions. Therefore, it successfully addresses the problems of low heating and cooling rates. In this paper, a soda-lime glass substrate coated with a DFR of the fluid to cycle through different temperature zones was used to form a micro-channel structure and integrated Pt heater continuous-flow PCR.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages487-490
Number of pages4
DOIs
StatePublished - 2011
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan, Province of China
Duration: 20 Feb 201123 Feb 2011

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period20/02/1123/02/11

Keywords

  • Bio chip
  • DNA
  • Dry Film Resist
  • Lab on a chip
  • PCR chip

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