Factors influencing in vitro shoot regeneration from leaf segments of Chrysanthemum

Aung Htay Naing, Su Min Jeon, Jeung Sul Han, Sun Hyung Lim, Ki Byung Lim, Chang Kil Kim

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The objective of this research was to develop an efficient protocol for shoot regeneration from leaf segments of the Chrysanthemum cv. Vivid Scarlet by examining the effects of plant growth regulators, dark incubation period, gelling agents, and silver nitrate. The highest number of shoots per explant (12.3) was regenerated from leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a combination of 1 mg L-1 of 6-benzyladenine (BA) and 2 mg L-1 of α-naphthaleneacetic acid (NAA) under light conditions without any initial dark period. Gelrite was the most effective gelling agent for shoot regeneration among those tested, whereas the presence of silver nitrate distinctly inhibited shoot regeneration. Superior plant growth and rooting was observed on a hormone-free MS medium solidified with Gelrite. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The established protocol was applicable to shoot regeneration for four out of six cultivars tested. This research will facilitate the genetic transformation and micropropagation of Chrysanthemum cultivars.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalComptes Rendus - Biologies
Volume337
Issue number6
DOIs
StatePublished - Jun 2014

Keywords

  • Flow cytometry
  • Gelling agent
  • Ornamental plant
  • Plant growth regulator
  • Silver nitrate

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