FeS₂-incorporated 3D PCL scaffold improves new bone formation and neovascularization in a rat calvarial defect model

Three-dimensional (3D) scaffolds composed of various biomaterials, including metals, ceramics, and synthetic polymers, have been widely used to regenerate bone defects. However, these materials possess clear downsides, which prevent bone regeneration. Therefore, composite scaffolds have been developed to compensate these disadvantages and achieve synergetic effects. In this study, a naturally occurring biomineral, FeS₂, was incorporated in PCL scaffolds to enhance the mechanical properties, which would in turn influence the biological characteristics. The composite scaffolds consisting of different weight fractions of FeS₂ were 3D printed and compared to pure PCL scaffold. The surface roughness (5.77-fold) and the compressive strength (3.38-fold) of the PCL scaffold was remarkably enhanced in a dose-dependent manner. The in vivo results showed that the group with PCL/FeS₂ scaffold implanted had increased neovascularization and bone formation (2.9-fold). These results demonstrated that the FeS₂ incorporated PCL scaffold might be an effective bioimplant for bone tissue regeneration.