TY - JOUR
T1 - Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells
AU - Li, Wei
AU - Sun, Ya Nan
AU - Yan, Xi Tao
AU - Yang, Seo Young
AU - Kim, Sohyun
AU - Lee, Young Mi
AU - Koh, Young Sang
AU - Kim, Young Ho
PY - 2014/2
Y1 - 2014/2
N2 - Radix Astragali (Astragalus membranaceus) is an important traditional Chinese medicine that is widely used as a tonic to enhance the body's natural defense mechanisms. In this phytochemical study, 12 flavonoids, isoliquiritigenin (1), liquiritigenin (2), calycosin (3), calycosin 7-O-β-d-glucoside (4), formononetin (5), formononetin 7-O-β-d- glucoside (6), daidzein (7), daidzein 7-O-β-d-glucoside (8), methylnissolin (9), methylnissolin 3-O-β-d-glucoside (10), isomucronulatol (11), and isomucronulatol 7-O-β-d-glucoside (12), were isolated from the roots of A. membranaceus. Their structures were elucidated by comparing spectroscopic data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1 and 2 exhibited significant inhibitory effects on LPS-induced IL-6 and IL-12 p40 production, with IC50 values ranging from 2.7 to 6.1 μM. Compound 1 also showed a moderate inhibitory effect on LPS-stimulated production of TNF-α with an IC50 value of 20.1 μM. Further studies of the potential anti-inflammatory effects and benefits of flavonoids from A. membranaceus are warranted.
AB - Radix Astragali (Astragalus membranaceus) is an important traditional Chinese medicine that is widely used as a tonic to enhance the body's natural defense mechanisms. In this phytochemical study, 12 flavonoids, isoliquiritigenin (1), liquiritigenin (2), calycosin (3), calycosin 7-O-β-d-glucoside (4), formononetin (5), formononetin 7-O-β-d- glucoside (6), daidzein (7), daidzein 7-O-β-d-glucoside (8), methylnissolin (9), methylnissolin 3-O-β-d-glucoside (10), isomucronulatol (11), and isomucronulatol 7-O-β-d-glucoside (12), were isolated from the roots of A. membranaceus. Their structures were elucidated by comparing spectroscopic data with reported values. The effects of the isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1 and 2 exhibited significant inhibitory effects on LPS-induced IL-6 and IL-12 p40 production, with IC50 values ranging from 2.7 to 6.1 μM. Compound 1 also showed a moderate inhibitory effect on LPS-stimulated production of TNF-α with an IC50 value of 20.1 μM. Further studies of the potential anti-inflammatory effects and benefits of flavonoids from A. membranaceus are warranted.
KW - Anti-inflammatory
KW - Astragalus membranaceus
KW - Flavonoid
KW - Leguminosae
UR - http://www.scopus.com/inward/record.url?scp=84894082617&partnerID=8YFLogxK
U2 - 10.1007/s12272-013-0174-7
DO - 10.1007/s12272-013-0174-7
M3 - Article
C2 - 23771500
AN - SCOPUS:84894082617
SN - 0253-6269
VL - 37
SP - 186
EP - 192
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 2
ER -