Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells

Sabina Shrestha; Dae Young Lee; Ji Hae Park; Jin Gyeong Cho; Dong Sung Lee; Bin Li; Youn Chul Kim; Yong Jin Jeon; Seung Woo Yeon; Nam In Baek

doi:10.1007/s10068-013-0161-2

Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells

Sabina Shrestha, Dae Young Lee, Ji Hae Park, Jin Gyeong Cho, Dong Sung Lee, Bin Li, Youn Chul Kim, Yong Jin Jeon, Seung Woo Yeon, Nam In Baek

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc) fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds, flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC₅₀ 3.5 μM), was the most potent neuroprotector with an EC₅₀ value of 11.90 μM.

Original language	English
Pages (from-to)	895-902
Number of pages	8
Journal	Food Science and Biotechnology
Volume	22
Issue number	4
DOIs	https://doi.org/10.1007/s10068-013-0161-2
State	Published - Aug 2013

Keywords

aureusidin
Ayurveda
Cdk5/p25
neuroprotection
Rhus parviflora

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10.1007/s10068-013-0161-2

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title = "Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells",

abstract = "Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc) fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds, flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC50 3.5 μM), was the most potent neuroprotector with an EC50 value of 11.90 μM.",

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author = "Sabina Shrestha and Lee, {Dae Young} and Park, {Ji Hae} and Cho, {Jin Gyeong} and Lee, {Dong Sung} and Bin Li and Kim, {Youn Chul} and Jeon, {Yong Jin} and Yeon, {Seung Woo} and Baek, {Nam In}",

year = "2013",

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T1 - Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells

AU - Shrestha, Sabina

AU - Lee, Dae Young

AU - Park, Ji Hae

AU - Cho, Jin Gyeong

AU - Lee, Dong Sung

AU - Li, Bin

AU - Kim, Youn Chul

AU - Jeon, Yong Jin

AU - Yeon, Seung Woo

AU - Baek, Nam In

PY - 2013/8

Y1 - 2013/8

N2 - Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc) fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds, flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC50 3.5 μM), was the most potent neuroprotector with an EC50 value of 11.90 μM.

AB - Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc) fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds, flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC50 3.5 μM), was the most potent neuroprotector with an EC50 value of 11.90 μM.

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Flavonoids from the fruits of Nepalese sumac (Rhus parviflora) attenuate glutamate-induced neurotoxicity in HT22 cells

Abstract

Keywords

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