Fluorene-Labeled 2'-Deoxyuridine as an Environmentally Sensitive Probe for Detection of an Abasic Site

Seung Woo Hong, So Young Lee, Gil Tae Hwang

Research output: Contribution to journalArticlepeer-review

Abstract

The fluorescence properties of the fluorene-labeled 2'-deoxyuridine derivative UF are sensitive to the polarity and viscosity of its solvent. We incorporated this environment-sensitive unit into the central position of oligodeoxynucleotides (ODNs) and placed various flanking bases (FBs) around the UF residue, then investigated the changes in fluorescence that occurred when these ODN probes encountered DNA targets containing an abasic site. The ODN probe featuring cytosine-FBs turned on its fluorescence when contacting the fully matched target DNA sequence, but turned off its fluorescence dramatically when the target DNA contained an abasic site.

Original languageEnglish
Pages (from-to)14480-14483
Number of pages4
JournalChemistrySelect
Volume5
Issue number45
DOIs
StatePublished - 7 Dec 2020

Keywords

  • abasic site
  • DNA
  • fluorene
  • fluorescent probes
  • oligonucleotides

Fingerprint

Dive into the research topics of 'Fluorene-Labeled 2'-Deoxyuridine as an Environmentally Sensitive Probe for Detection of an Abasic Site'. Together they form a unique fingerprint.

Cite this