Abstract
The fluorescence properties of the fluorene-labeled 2'-deoxyuridine derivative UF are sensitive to the polarity and viscosity of its solvent. We incorporated this environment-sensitive unit into the central position of oligodeoxynucleotides (ODNs) and placed various flanking bases (FBs) around the UF residue, then investigated the changes in fluorescence that occurred when these ODN probes encountered DNA targets containing an abasic site. The ODN probe featuring cytosine-FBs turned on its fluorescence when contacting the fully matched target DNA sequence, but turned off its fluorescence dramatically when the target DNA contained an abasic site.
Original language | English |
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Pages (from-to) | 14480-14483 |
Number of pages | 4 |
Journal | ChemistrySelect |
Volume | 5 |
Issue number | 45 |
DOIs | |
State | Published - 7 Dec 2020 |
Keywords
- abasic site
- DNA
- fluorene
- fluorescent probes
- oligonucleotides