Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)-2′- deoxyuridine: Sequence-specific fluorescence changes upon duplex formation

Gil Tae Hwang; Young Jun Seo; Su Jeong Kim; Byeang Hyean Kim

doi:10.1016/j.tetlet.2004.03.071

Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)-2′- deoxyuridine: Sequence-specific fluorescence changes upon duplex formation

Gil Tae Hwang, Young Jun Seo, Su Jeong Kim, Byeang Hyean Kim

Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity.

Original language	English
Pages (from-to)	3543-3546
Number of pages	4
Journal	Tetrahedron Letters
Volume	45
Issue number	18
DOIs	https://doi.org/10.1016/j.tetlet.2004.03.071
State	Published - 26 Apr 2004

Keywords

Fluorescence
Nucleosides
Nucleotides

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10.1016/j.tetlet.2004.03.071

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abstract = "We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity.",

keywords = "Fluorescence, Nucleosides, Nucleotides",

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AU - Hwang, Gil Tae

AU - Seo, Young Jun

AU - Kim, Su Jeong

AU - Kim, Byeang Hyean

PY - 2004/4/26

Y1 - 2004/4/26

N2 - We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity.

AB - We describe the design and properties of a pyrene-labeled deoxyuridine that can be inserted efficiently into oligodeoxynucleotides using phosphoramidite chemistry. An oligonucleotide incorporating the pyrene-labeled deoxyuridine is a sensitive fluorescence probe that can discriminate between perfect and single-base-mismatched pairing by changes in its fluorescence intensity.

KW - Fluorescence

KW - Nucleosides

KW - Nucleotides

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DO - 10.1016/j.tetlet.2004.03.071

M3 - Article

AN - SCOPUS:1842737324

SN - 0040-4039

VL - 45

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JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 18

ER -

Fluorescent oligonucleotide incorporating 5-(1-ethynylpyrenyl)-2′- deoxyuridine: Sequence-specific fluorescence changes upon duplex formation

Abstract

Keywords

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