Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation

Yu Jin Jung; Ran Kim; Hyun Joo Ham; Sang In Park; Min Young Lee; Jongmin Kim; Jihwan Hwang; Moon Seo Park; Seung Schik Yoo; Lee So Maeng; Woochul Chang; Yong An Chung

doi:10.1016/j.ultrasmedbio.2014.11.008

Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation

Yu Jin Jung, Ran Kim, Hyun Joo Ham, Sang In Park, Min Young Lee, Jongmin Kim, Jihwan Hwang, Moon Seo Park, Seung Schik Yoo, Lee So Maeng, Woochul Chang, Yong An Chung

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.

Original language	English
Pages (from-to)	999-1007
Number of pages	9
Journal	Ultrasound in Medicine and Biology
Volume	41
Issue number	4
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2014.11.008
State	Published - 1 Apr 2015

Keywords

Bone regeneration
Calvarial bone fracture
Focused low-intensity pulsed ultrasound
Osteocyte proliferation

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10.1016/j.ultrasmedbio.2014.11.008

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Jung, Y. J., Kim, R., Ham, H. J., Park, S. I., Lee, M. Y., Kim, J., Hwang, J., Park, M. S., Yoo, S. S., Maeng, L. S., Chang, W., & Chung, Y. A. (2015). Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation. Ultrasound in Medicine and Biology, 41(4), 999-1007. https://doi.org/10.1016/j.ultrasmedbio.2014.11.008

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title = "Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation",

abstract = "A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.",

keywords = "Bone regeneration, Calvarial bone fracture, Focused low-intensity pulsed ultrasound, Osteocyte proliferation",

author = "Jung, {Yu Jin} and Ran Kim and Ham, {Hyun Joo} and Park, {Sang In} and Lee, {Min Young} and Jongmin Kim and Jihwan Hwang and Park, {Moon Seo} and Yoo, {Seung Schik} and Maeng, {Lee So} and Woochul Chang and Chung, {Yong An}",

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Jung, YJ, Kim, R, Ham, HJ, Park, SI, Lee, MY, Kim, J, Hwang, J, Park, MS, Yoo, SS, Maeng, LS, Chang, W & Chung, YA 2015, 'Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation', Ultrasound in Medicine and Biology, vol. 41, no. 4, pp. 999-1007. https://doi.org/10.1016/j.ultrasmedbio.2014.11.008

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AU - Jung, Yu Jin

AU - Kim, Ran

AU - Ham, Hyun Joo

AU - Park, Sang In

AU - Lee, Min Young

AU - Kim, Jongmin

AU - Hwang, Jihwan

AU - Park, Moon Seo

AU - Yoo, Seung Schik

AU - Maeng, Lee So

AU - Chang, Woochul

AU - Chung, Yong An

PY - 2015/4/1

Y1 - 2015/4/1

N2 - A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.

AB - A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.

KW - Bone regeneration

KW - Calvarial bone fracture

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Focused low-intensity pulsed ultrasound enhances bone regeneration in rat calvarial bone defect through enhancement of cell proliferation

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