Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family

Youn Kyoung Goo; Gabriel Oluga Aboge; M. Alaa Terkawi; Honglin Jia; Junya Yamagishi; Fujiko Sunaga; Kazuhiko Namikawa; Se Yeoun Cha; Hyung Kwan Jang; Suk Kim; Yoshifumi Nishikawa; Xuenan Xuan

doi:10.1016/j.parint.2011.11.007

Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family

Youn Kyoung Goo, Gabriel Oluga Aboge, M. Alaa Terkawi, Honglin Jia, Junya Yamagishi, Fujiko Sunaga, Kazuhiko Namikawa, Se Yeoun Cha, Hyung Kwan Jang, Suk Kim, Yoshifumi Nishikawa, Xuenan Xuan

Department of Medicine

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8 Scopus citations

Abstract

We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high sensitivity, and ELISA with rBgP32 resulted in high specificity and in concordance with IFAT results.

Original language	English
Pages (from-to)	364-368
Number of pages	5
Journal	Parasitology International
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/j.parint.2011.11.007
State	Published - Jun 2012

Keywords

Babesia gibsoni
ELISA
Merozoite surface protein

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.parint.2011.11.007

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Goo, Y. K., Aboge, G. O., Terkawi, M. A., Jia, H., Yamagishi, J., Sunaga, F., Namikawa, K., Cha, S. Y., Jang, H. K., Kim, S., Nishikawa, Y., & Xuan, X. (2012). Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family. Parasitology International, 61(2), 364-368. https://doi.org/10.1016/j.parint.2011.11.007

@article{d047a43f838947c8b933746027e62939,

title = "Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family",

abstract = "We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high sensitivity, and ELISA with rBgP32 resulted in high specificity and in concordance with IFAT results.",

keywords = "Babesia gibsoni, ELISA, Merozoite surface protein",

author = "Goo, {Youn Kyoung} and Aboge, {Gabriel Oluga} and Terkawi, {M. Alaa} and Honglin Jia and Junya Yamagishi and Fujiko Sunaga and Kazuhiko Namikawa and Cha, {Se Yeoun} and Jang, {Hyung Kwan} and Suk Kim and Yoshifumi Nishikawa and Xuenan Xuan",

year = "2012",

month = jun,

doi = "10.1016/j.parint.2011.11.007",

language = "English",

volume = "61",

pages = "364--368",

journal = "Parasitology International",

issn = "1383-5769",

publisher = "Elsevier Ireland Ltd",

number = "2",

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Goo, YK, Aboge, GO, Terkawi, MA, Jia, H, Yamagishi, J, Sunaga, F, Namikawa, K, Cha, SY, Jang, HK, Kim, S, Nishikawa, Y & Xuan, X 2012, 'Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family', Parasitology International, vol. 61, no. 2, pp. 364-368. https://doi.org/10.1016/j.parint.2011.11.007

Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family. / Goo, Youn Kyoung; Aboge, Gabriel Oluga; Terkawi, M. Alaa et al.
In: Parasitology International, Vol. 61, No. 2, 06.2012, p. 364-368.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family

AU - Goo, Youn Kyoung

AU - Aboge, Gabriel Oluga

AU - Terkawi, M. Alaa

AU - Jia, Honglin

AU - Yamagishi, Junya

AU - Sunaga, Fujiko

AU - Namikawa, Kazuhiko

AU - Cha, Se Yeoun

AU - Jang, Hyung Kwan

AU - Kim, Suk

AU - Nishikawa, Yoshifumi

AU - Xuan, Xuenan

PY - 2012/6

Y1 - 2012/6

N2 - We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high sensitivity, and ELISA with rBgP32 resulted in high specificity and in concordance with IFAT results.

AB - We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding native proteins belong to merozoite surface protein family of B. gibsoni (BgMSP). Moreover, antisera against either rBgP45 or rBgP47 cross-reacted with all the proteins of the BgMSP family on ELISA and IFAT analyses. Of the four candidate antigens, ELISA with rBgP45 yielded high sensitivity, and ELISA with rBgP32 resulted in high specificity and in concordance with IFAT results.

KW - Babesia gibsoni

KW - ELISA

KW - Merozoite surface protein

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DO - 10.1016/j.parint.2011.11.007

M3 - Article

C2 - 22172478

AN - SCOPUS:84857798297

SN - 1383-5769

VL - 61

SP - 364

EP - 368

JO - Parasitology International

JF - Parasitology International

IS - 2

ER -

Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family

Abstract

Keywords

UN SDGs

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