Functional expression of RuBisCO reduces CO2 emission during fermentation by engineered Saccharomyces cerevisiae

Sujeong Park; Bo Ram Park; Deokyeol Jeong; Jongbeom Park; Ja Kyong Ko; Soo Jung Kim; Jeong Sun Kim; Yong Su Jin; Soo Rin Kim

doi:10.1016/j.procbio.2023.10.013

Functional expression of RuBisCO reduces CO₂ emission during fermentation by engineered Saccharomyces cerevisiae

Sujeong Park
, Bo Ram Park
, Deokyeol Jeong
, Jongbeom Park
, Ja Kyong Ko
, Soo Jung Kim
, Jeong Sun Kim
, Yong Su Jin
, Soo Rin Kim

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Industrial biotechnology based on yeast fermentation is a promising strategy that can alleviate global warming and climate change. However, Saccharomyces cerevisiae, widely used in bioprocesses, releases a large amount of carbon dioxide (CO₂) during fermentation. This study developed a mixotrophic CO₂-fixing S. cerevisiae to achieve carbon neutrality and sustainability in bioprocess. A CO₂-fixation pathway was constructed in a xylose-utilizing S. cerevisiae by heterologous expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) and phosphoribulokinase (PRK). Furthermore, a delta-integration strategy was utilized, and the RuBisCO gene copy number was increased to 10 copies to improve the efficiency of CO₂-fixation. An additional Cas9-based genome editing was performed to overexpress other CO₂-fixation related genes. The resulting CO₂-fixing yeast, SJ03, exhibited the highest RuBisCO activity. During anaerobic xylose fermentation, ethanol concentration was increased by 17% and ethanol yield was increased by 16% compared to the control strain. In addition, CO₂ emissions decreased by 7%. These results suggest that overexpression of the CO₂-fixation pathway coupled with xylose utilization in S. cerevisiae might reduce CO₂ emission in bioprocesses.

Original language	English
Pages (from-to)	286-293
Number of pages	8
Journal	Process Biochemistry
Volume	134
DOIs	https://doi.org/10.1016/j.procbio.2023.10.013
State	Published - Nov 2023

Keywords

5-bisphosphate carboxylase/oxygenase
CRISPR/Cas9
Carbon dioxide fixation
Delta-integration strategy
Ribulose-1
Saccharomyces cerevisiae

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.procbio.2023.10.013

Cite this

@article{2156d8ac76da4c279cdf2f922dde319c,

title = "Functional expression of RuBisCO reduces CO2 emission during fermentation by engineered Saccharomyces cerevisiae",

abstract = "Industrial biotechnology based on yeast fermentation is a promising strategy that can alleviate global warming and climate change. However, Saccharomyces cerevisiae, widely used in bioprocesses, releases a large amount of carbon dioxide (CO2) during fermentation. This study developed a mixotrophic CO2-fixing S. cerevisiae to achieve carbon neutrality and sustainability in bioprocess. A CO2-fixation pathway was constructed in a xylose-utilizing S. cerevisiae by heterologous expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) and phosphoribulokinase (PRK). Furthermore, a delta-integration strategy was utilized, and the RuBisCO gene copy number was increased to 10 copies to improve the efficiency of CO2-fixation. An additional Cas9-based genome editing was performed to overexpress other CO2-fixation related genes. The resulting CO2-fixing yeast, SJ03, exhibited the highest RuBisCO activity. During anaerobic xylose fermentation, ethanol concentration was increased by 17\% and ethanol yield was increased by 16\% compared to the control strain. In addition, CO2 emissions decreased by 7\%. These results suggest that overexpression of the CO2-fixation pathway coupled with xylose utilization in S. cerevisiae might reduce CO2 emission in bioprocesses.",

keywords = "5-bisphosphate carboxylase/oxygenase, CRISPR/Cas9, Carbon dioxide fixation, Delta-integration strategy, Ribulose-1, Saccharomyces cerevisiae",

author = "Sujeong Park and Park, \{Bo Ram\} and Deokyeol Jeong and Jongbeom Park and Ko, \{Ja Kyong\} and Kim, \{Soo Jung\} and Kim, \{Jeong Sun\} and Jin, \{Yong Su\} and Kim, \{Soo Rin\}",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Ltd",

year = "2023",

month = nov,

doi = "10.1016/j.procbio.2023.10.013",

language = "English",

volume = "134",

pages = "286--293",

journal = "Process Biochemistry",

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AU - Park, Sujeong

AU - Park, Bo Ram

AU - Jeong, Deokyeol

AU - Park, Jongbeom

AU - Ko, Ja Kyong

AU - Kim, Soo Jung

AU - Kim, Jeong Sun

AU - Jin, Yong Su

AU - Kim, Soo Rin

PY - 2023/11

Y1 - 2023/11

N2 - Industrial biotechnology based on yeast fermentation is a promising strategy that can alleviate global warming and climate change. However, Saccharomyces cerevisiae, widely used in bioprocesses, releases a large amount of carbon dioxide (CO2) during fermentation. This study developed a mixotrophic CO2-fixing S. cerevisiae to achieve carbon neutrality and sustainability in bioprocess. A CO2-fixation pathway was constructed in a xylose-utilizing S. cerevisiae by heterologous expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) and phosphoribulokinase (PRK). Furthermore, a delta-integration strategy was utilized, and the RuBisCO gene copy number was increased to 10 copies to improve the efficiency of CO2-fixation. An additional Cas9-based genome editing was performed to overexpress other CO2-fixation related genes. The resulting CO2-fixing yeast, SJ03, exhibited the highest RuBisCO activity. During anaerobic xylose fermentation, ethanol concentration was increased by 17% and ethanol yield was increased by 16% compared to the control strain. In addition, CO2 emissions decreased by 7%. These results suggest that overexpression of the CO2-fixation pathway coupled with xylose utilization in S. cerevisiae might reduce CO2 emission in bioprocesses.

AB - Industrial biotechnology based on yeast fermentation is a promising strategy that can alleviate global warming and climate change. However, Saccharomyces cerevisiae, widely used in bioprocesses, releases a large amount of carbon dioxide (CO2) during fermentation. This study developed a mixotrophic CO2-fixing S. cerevisiae to achieve carbon neutrality and sustainability in bioprocess. A CO2-fixation pathway was constructed in a xylose-utilizing S. cerevisiae by heterologous expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) and phosphoribulokinase (PRK). Furthermore, a delta-integration strategy was utilized, and the RuBisCO gene copy number was increased to 10 copies to improve the efficiency of CO2-fixation. An additional Cas9-based genome editing was performed to overexpress other CO2-fixation related genes. The resulting CO2-fixing yeast, SJ03, exhibited the highest RuBisCO activity. During anaerobic xylose fermentation, ethanol concentration was increased by 17% and ethanol yield was increased by 16% compared to the control strain. In addition, CO2 emissions decreased by 7%. These results suggest that overexpression of the CO2-fixation pathway coupled with xylose utilization in S. cerevisiae might reduce CO2 emission in bioprocesses.

KW - 5-bisphosphate carboxylase/oxygenase

KW - CRISPR/Cas9

KW - Carbon dioxide fixation

KW - Delta-integration strategy

KW - Ribulose-1

KW - Saccharomyces cerevisiae

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