Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice

Seong Kyu Han; Seon Ah Park; Jae Gyu Jeon; Kee Wan Chang; Dong Kuk Ahn; Seok Bae Oh; Soo Joung Park

doi:10.1016/j.neulet.2009.01.061

Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice

Seong Kyu Han, Seon Ah Park, Jae Gyu Jeon, Kee Wan Chang, Dong Kuk Ahn, Seok Bae Oh, Soo Joung Park

Department of Dentistry

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The aim of this study was to investigate the existence of functional TRPV1 receptor by substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc), which is implicated in the processing of nociceptive information from orofacial regions. The direct membrane effects of a TRPV1 receptor agonist, capsaicin, were examined by gramicidin-perforated patch clamp recording using a trigeminal brainstem slice preparation containing Vc from immature mice. Capsaicin (1-2 μM) induced a membrane depolarization in 58 out of 71 SG neurons tested (82%). Capsaicin-induced depolarization was maintained in 20 out of 32 (63%) SG neurons in the presence of amino acid and voltage-dependent sodium channel blockers (10 μM CNQX, 20 μM AP-5, 0.5 μM TTX, 50 μM picrotoxin and 2 μM strychnine). In addition, capsaicin-induced depolarization was maintained in the presence of L-732,138 (1 μM), an NK1 receptor antagonist, in 14 out of 17 neurons (82%) tested. The capsaicin-induced depolarizing effects were blocked by a TRPV1 receptor antagonist, capsazepine (10 μM). These results indicate that a sub-population of SG neurons in the Vc express functional TRPV1 receptors, and that capsaicin can directly activate the TRPV1 receptor on the postsynaptic membrane of SG neurons.

Original language	English
Pages (from-to)	228-231
Number of pages	4
Journal	Neuroscience Letters
Volume	452
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2009.01.061
State	Published - 20 Mar 2009

Keywords

Capsaicin
Patch clamp
Substantia gelatinosa of trigeminal brainstem nucleus
TRPV1 receptor

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10.1016/j.neulet.2009.01.061

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title = "Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice",

abstract = "The aim of this study was to investigate the existence of functional TRPV1 receptor by substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc), which is implicated in the processing of nociceptive information from orofacial regions. The direct membrane effects of a TRPV1 receptor agonist, capsaicin, were examined by gramicidin-perforated patch clamp recording using a trigeminal brainstem slice preparation containing Vc from immature mice. Capsaicin (1-2 μM) induced a membrane depolarization in 58 out of 71 SG neurons tested (82%). Capsaicin-induced depolarization was maintained in 20 out of 32 (63%) SG neurons in the presence of amino acid and voltage-dependent sodium channel blockers (10 μM CNQX, 20 μM AP-5, 0.5 μM TTX, 50 μM picrotoxin and 2 μM strychnine). In addition, capsaicin-induced depolarization was maintained in the presence of L-732,138 (1 μM), an NK1 receptor antagonist, in 14 out of 17 neurons (82%) tested. The capsaicin-induced depolarizing effects were blocked by a TRPV1 receptor antagonist, capsazepine (10 μM). These results indicate that a sub-population of SG neurons in the Vc express functional TRPV1 receptors, and that capsaicin can directly activate the TRPV1 receptor on the postsynaptic membrane of SG neurons.",

keywords = "Capsaicin, Patch clamp, Substantia gelatinosa of trigeminal brainstem nucleus, TRPV1 receptor",

author = "Han, {Seong Kyu} and Park, {Seon Ah} and Jeon, {Jae Gyu} and Chang, {Kee Wan} and Ahn, {Dong Kuk} and Oh, {Seok Bae} and Park, {Soo Joung}",

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T1 - Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice

AU - Han, Seong Kyu

AU - Park, Seon Ah

AU - Jeon, Jae Gyu

AU - Chang, Kee Wan

AU - Ahn, Dong Kuk

AU - Oh, Seok Bae

AU - Park, Soo Joung

PY - 2009/3/20

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N2 - The aim of this study was to investigate the existence of functional TRPV1 receptor by substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc), which is implicated in the processing of nociceptive information from orofacial regions. The direct membrane effects of a TRPV1 receptor agonist, capsaicin, were examined by gramicidin-perforated patch clamp recording using a trigeminal brainstem slice preparation containing Vc from immature mice. Capsaicin (1-2 μM) induced a membrane depolarization in 58 out of 71 SG neurons tested (82%). Capsaicin-induced depolarization was maintained in 20 out of 32 (63%) SG neurons in the presence of amino acid and voltage-dependent sodium channel blockers (10 μM CNQX, 20 μM AP-5, 0.5 μM TTX, 50 μM picrotoxin and 2 μM strychnine). In addition, capsaicin-induced depolarization was maintained in the presence of L-732,138 (1 μM), an NK1 receptor antagonist, in 14 out of 17 neurons (82%) tested. The capsaicin-induced depolarizing effects were blocked by a TRPV1 receptor antagonist, capsazepine (10 μM). These results indicate that a sub-population of SG neurons in the Vc express functional TRPV1 receptors, and that capsaicin can directly activate the TRPV1 receptor on the postsynaptic membrane of SG neurons.

AB - The aim of this study was to investigate the existence of functional TRPV1 receptor by substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc), which is implicated in the processing of nociceptive information from orofacial regions. The direct membrane effects of a TRPV1 receptor agonist, capsaicin, were examined by gramicidin-perforated patch clamp recording using a trigeminal brainstem slice preparation containing Vc from immature mice. Capsaicin (1-2 μM) induced a membrane depolarization in 58 out of 71 SG neurons tested (82%). Capsaicin-induced depolarization was maintained in 20 out of 32 (63%) SG neurons in the presence of amino acid and voltage-dependent sodium channel blockers (10 μM CNQX, 20 μM AP-5, 0.5 μM TTX, 50 μM picrotoxin and 2 μM strychnine). In addition, capsaicin-induced depolarization was maintained in the presence of L-732,138 (1 μM), an NK1 receptor antagonist, in 14 out of 17 neurons (82%) tested. The capsaicin-induced depolarizing effects were blocked by a TRPV1 receptor antagonist, capsazepine (10 μM). These results indicate that a sub-population of SG neurons in the Vc express functional TRPV1 receptors, and that capsaicin can directly activate the TRPV1 receptor on the postsynaptic membrane of SG neurons.

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KW - Patch clamp

KW - Substantia gelatinosa of trigeminal brainstem nucleus

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Functional type I vanilloid receptor expression by substantia gelatinosa neurons of trigeminal subnucleus caudalis in mice

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