Fungicidal effect of indolicidin and its interaction with phospholipid membranes

Dong Gun Lee; Hyung Keun Kim; Sun Am Kim; Yoonkyung Park; Seong Cheol Park; Seung Hwan Jang; Kyung Soo Hahm

doi:10.1016/S0006-291X(03)00755-1

Fungicidal effect of indolicidin and its interaction with phospholipid membranes

Dong Gun Lee, Hyung Keun Kim, Sun Am Kim, Yoonkyung Park, Seong Cheol Park, Seung Hwan Jang, Kyung Soo Hahm

Chosun University

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

The fungicidal effect and mechanism of a tryptophan-rich 13-mer peptide, indolicidin derived from granules of bovine neutrophils, were investigated. Indolicidin displayed a strong fungicidal activity against various fungi. In order to understand the fungicidal mechanism(s) of indolicidin, we examined the interaction of indolicidin with the pathogenic fungus Trichosporon beigelii. Fluorescence confocal microscopy and flow cytometry analysis revealed that indolicidin acted rapidly on the plasma membrane of the fungal cells in an energy-independent manner. This interaction is also dependent on the ionic environment. Furthermore, indolicidin caused significant morphological changes when tested for the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w). The results suggest that indolicidin may exert its fungicidal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers, in a salt-dependent and energy-independent manner.

Original language	English
Pages (from-to)	305-310
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	305
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(03)00755-1
State	Published - 30 May 2003

Keywords

Fungicidal mechanism
Indolicidin
Liposomes
Tryptophan-rich 13-mer peptide

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10.1016/S0006-291X(03)00755-1

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title = "Fungicidal effect of indolicidin and its interaction with phospholipid membranes",

abstract = "The fungicidal effect and mechanism of a tryptophan-rich 13-mer peptide, indolicidin derived from granules of bovine neutrophils, were investigated. Indolicidin displayed a strong fungicidal activity against various fungi. In order to understand the fungicidal mechanism(s) of indolicidin, we examined the interaction of indolicidin with the pathogenic fungus Trichosporon beigelii. Fluorescence confocal microscopy and flow cytometry analysis revealed that indolicidin acted rapidly on the plasma membrane of the fungal cells in an energy-independent manner. This interaction is also dependent on the ionic environment. Furthermore, indolicidin caused significant morphological changes when tested for the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w). The results suggest that indolicidin may exert its fungicidal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers, in a salt-dependent and energy-independent manner.",

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AU - Lee, Dong Gun

AU - Kim, Hyung Keun

AU - Kim, Sun Am

AU - Park, Yoonkyung

AU - Park, Seong Cheol

AU - Jang, Seung Hwan

AU - Hahm, Kyung Soo

PY - 2003/5/30

Y1 - 2003/5/30

N2 - The fungicidal effect and mechanism of a tryptophan-rich 13-mer peptide, indolicidin derived from granules of bovine neutrophils, were investigated. Indolicidin displayed a strong fungicidal activity against various fungi. In order to understand the fungicidal mechanism(s) of indolicidin, we examined the interaction of indolicidin with the pathogenic fungus Trichosporon beigelii. Fluorescence confocal microscopy and flow cytometry analysis revealed that indolicidin acted rapidly on the plasma membrane of the fungal cells in an energy-independent manner. This interaction is also dependent on the ionic environment. Furthermore, indolicidin caused significant morphological changes when tested for the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w). The results suggest that indolicidin may exert its fungicidal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers, in a salt-dependent and energy-independent manner.

AB - The fungicidal effect and mechanism of a tryptophan-rich 13-mer peptide, indolicidin derived from granules of bovine neutrophils, were investigated. Indolicidin displayed a strong fungicidal activity against various fungi. In order to understand the fungicidal mechanism(s) of indolicidin, we examined the interaction of indolicidin with the pathogenic fungus Trichosporon beigelii. Fluorescence confocal microscopy and flow cytometry analysis revealed that indolicidin acted rapidly on the plasma membrane of the fungal cells in an energy-independent manner. This interaction is also dependent on the ionic environment. Furthermore, indolicidin caused significant morphological changes when tested for the membrane disrupting activity using liposomes (phosphatidylcholine/cholesterol; 10:1, w/w). The results suggest that indolicidin may exert its fungicidal activity by disrupting the structure of cell membranes, via direct interaction with the lipid bilayers, in a salt-dependent and energy-independent manner.

KW - Fungicidal mechanism

KW - Indolicidin

KW - Liposomes

KW - Tryptophan-rich 13-mer peptide

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JF - Biochemical and Biophysical Research Communications

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Fungicidal effect of indolicidin and its interaction with phospholipid membranes

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Keywords

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