TY - JOUR
T1 - Fusarium proliferatum KGL0401 as a new gibberellin-producing fungus
AU - Rim, Soon Ok
AU - Lee, Jin Hyung
AU - Choi, Wha Youl
AU - Hwang, Seon Kap
AU - Suh, Seok Jong
AU - Lee, In Joong
AU - Rhee, In Koo
AU - Kim, Jong Guk
PY - 2005/8
Y1 - 2005/8
N2 - Gibberellins (GAs) play an important role in plant growth and development. Fifteen fungi were isolated from Physalis alkekengi var francheti plant roots, and among them, four isolates showed GA-production activity. A bioassay using waito-c rice was carried out with the culture fluid of the GA-producing fungi. The GA-producing fungi were cultured for 7 days in Czapek's liquid medium at 30°C, 120 rpm, under dark conditions. The culture broth was concentrated 30-fold and 10 μl of that concentrate was applied to 2-leaf rice sprouts. The height of the rice seedlings treated with the culture fluid of isolate PA08 was 26 cm high, while that of the seedlings treated with the wild-type Gibberella fujikuroi was 13 cm high. As such, the plant growth-promoting activity exhibited by isolate PA08 was 2 times stronger than that exhibited by the wild-type G fujikuroi. The amounts of GA1, GA3, GA4, GA7, GA9, GA20, and GA24 in the medium were measured using gas chromatography-mass spectrometry (GC-MS), and the quantities produced by isolate PA08 were 4.85 ng/ml, 4.79 ng/ml, 17.30 ng/ml, 6.01 ng/ml, 16.61 ng/ml, 0.08 ng/ml, and 17.30 ng/ml, respectively. Isolate PA08 was also identified as Fusarium proliferatum KGL0401 by a genetic analysis of the nucleotide sequences of the internal transcribed spacer region of the ribosomal DNA.
AB - Gibberellins (GAs) play an important role in plant growth and development. Fifteen fungi were isolated from Physalis alkekengi var francheti plant roots, and among them, four isolates showed GA-production activity. A bioassay using waito-c rice was carried out with the culture fluid of the GA-producing fungi. The GA-producing fungi were cultured for 7 days in Czapek's liquid medium at 30°C, 120 rpm, under dark conditions. The culture broth was concentrated 30-fold and 10 μl of that concentrate was applied to 2-leaf rice sprouts. The height of the rice seedlings treated with the culture fluid of isolate PA08 was 26 cm high, while that of the seedlings treated with the wild-type Gibberella fujikuroi was 13 cm high. As such, the plant growth-promoting activity exhibited by isolate PA08 was 2 times stronger than that exhibited by the wild-type G fujikuroi. The amounts of GA1, GA3, GA4, GA7, GA9, GA20, and GA24 in the medium were measured using gas chromatography-mass spectrometry (GC-MS), and the quantities produced by isolate PA08 were 4.85 ng/ml, 4.79 ng/ml, 17.30 ng/ml, 6.01 ng/ml, 16.61 ng/ml, 0.08 ng/ml, and 17.30 ng/ml, respectively. Isolate PA08 was also identified as Fusarium proliferatum KGL0401 by a genetic analysis of the nucleotide sequences of the internal transcribed spacer region of the ribosomal DNA.
KW - Fusarium proliferation KGL0401
KW - Gibberellin
KW - Physalis alkekengi var. francheti
UR - http://www.scopus.com/inward/record.url?scp=24644504103&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:24644504103
SN - 1017-7825
VL - 15
SP - 809
EP - 814
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 4
ER -