Fusarium proliferatum KGL0401 as a new gibberellin-producing fungus

Soon Ok Rim, Jin Hyung Lee, Wha Youl Choi, Seon Kap Hwang, Seok Jong Suh, In Joong Lee, In Koo Rhee, Jong Guk Kim

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31 Scopus citations

Abstract

Gibberellins (GAs) play an important role in plant growth and development. Fifteen fungi were isolated from Physalis alkekengi var francheti plant roots, and among them, four isolates showed GA-production activity. A bioassay using waito-c rice was carried out with the culture fluid of the GA-producing fungi. The GA-producing fungi were cultured for 7 days in Czapek's liquid medium at 30°C, 120 rpm, under dark conditions. The culture broth was concentrated 30-fold and 10 μl of that concentrate was applied to 2-leaf rice sprouts. The height of the rice seedlings treated with the culture fluid of isolate PA08 was 26 cm high, while that of the seedlings treated with the wild-type Gibberella fujikuroi was 13 cm high. As such, the plant growth-promoting activity exhibited by isolate PA08 was 2 times stronger than that exhibited by the wild-type G fujikuroi. The amounts of GA1, GA3, GA4, GA7, GA9, GA20, and GA24 in the medium were measured using gas chromatography-mass spectrometry (GC-MS), and the quantities produced by isolate PA08 were 4.85 ng/ml, 4.79 ng/ml, 17.30 ng/ml, 6.01 ng/ml, 16.61 ng/ml, 0.08 ng/ml, and 17.30 ng/ml, respectively. Isolate PA08 was also identified as Fusarium proliferatum KGL0401 by a genetic analysis of the nucleotide sequences of the internal transcribed spacer region of the ribosomal DNA.

Original languageEnglish
Pages (from-to)809-814
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number4
StatePublished - Aug 2005

Keywords

  • Fusarium proliferation KGL0401
  • Gibberellin
  • Physalis alkekengi var. francheti

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