Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie

Jae Kwang Jin; Yeo Jung Na; Joon Ho Song; Hong Gu Joo; Seungjoon Kim; Jae Il Kim; Eun Kyoung Choi; Richard I. Carp; Yong Sun Kim; Taekyun Shin

doi:10.1016/j.neulet.2007.04.069

Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie

Jae Kwang Jin, Yeo Jung Na, Joon Ho Song, Hong Gu Joo, Seungjoon Kim, Jae Il Kim, Eun Kyoung Choi, Richard I. Carp, Yong Sun Kim, Taekyun Shin

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrP^Sc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrP^Sc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.

Original language	English
Pages (from-to)	138-143
Number of pages	6
Journal	Neuroscience Letters
Volume	420
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2007.04.069
State	Published - 13 Jun 2007

Keywords

Galectin-3
Macrophage
Microglia
Prion disease
Scrapie

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10.1016/j.neulet.2007.04.069

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title = "Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie",

abstract = "To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrPSc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrPSc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.",

keywords = "Galectin-3, Macrophage, Microglia, Prion disease, Scrapie",

author = "Jin, {Jae Kwang} and Na, {Yeo Jung} and Song, {Joon Ho} and Joo, {Hong Gu} and Seungjoon Kim and Kim, {Jae Il} and Choi, {Eun Kyoung} and Carp, {Richard I.} and Kim, {Yong Sun} and Taekyun Shin",

year = "2007",

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doi = "10.1016/j.neulet.2007.04.069",

language = "English",

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AU - Jin, Jae Kwang

AU - Na, Yeo Jung

AU - Song, Joon Ho

AU - Joo, Hong Gu

AU - Kim, Seungjoon

AU - Kim, Jae Il

AU - Choi, Eun Kyoung

AU - Carp, Richard I.

AU - Kim, Yong Sun

AU - Shin, Taekyun

PY - 2007/6/13

Y1 - 2007/6/13

N2 - To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrPSc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrPSc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.

AB - To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrPSc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrPSc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.

KW - Galectin-3

KW - Macrophage

KW - Microglia

KW - Prion disease

KW - Scrapie

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JF - Neuroscience Letters

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ER -

Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie

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Keywords

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