Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Eun Jung Chung; Young Kwan Sung; Mohammad Farooq; Younghee Kim; Sanguk Im; Won Young Tak; Yoon Jin Hwang; Yang Il Kim; Hyung Soo Han; Jung Chul Kim; Moon Kyu Kim

Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Eun Jung Chung, Young Kwan Sung, Mohammad Farooq, Younghee Kim, Sanguk Im, Won Young Tak, Yoon Jin Hwang, Yang Il Kim, Hyung Soo Han, Jung Chul Kim, Moon Kyu Kim

Department of Medicine

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

Original language	English
Pages (from-to)	382-387
Number of pages	6
Journal	Molecules and Cells
Volume	14
Issue number	3
State	Published - Dec 2002

Keywords

cDNA Microarray
Cluster
Hepatocellular Carcinoma
RT-PCR

Cite this

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title = "Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray",

abstract = "We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.",

keywords = "cDNA Microarray, Cluster, Hepatocellular Carcinoma, RT-PCR",

author = "Chung, {Eun Jung} and Sung, {Young Kwan} and Mohammad Farooq and Younghee Kim and Sanguk Im and Tak, {Won Young} and Hwang, {Yoon Jin} and Kim, {Yang Il} and Han, {Hyung Soo} and Kim, {Jung Chul} and Kim, {Moon Kyu}",

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language = "English",

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T1 - Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

AU - Chung, Eun Jung

AU - Sung, Young Kwan

AU - Farooq, Mohammad

AU - Kim, Younghee

AU - Im, Sanguk

AU - Tak, Won Young

AU - Hwang, Yoon Jin

AU - Kim, Yang Il

AU - Han, Hyung Soo

AU - Kim, Jung Chul

AU - Kim, Moon Kyu

PY - 2002/12

Y1 - 2002/12

N2 - We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

AB - We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

KW - cDNA Microarray

KW - Cluster

KW - Hepatocellular Carcinoma

KW - RT-PCR

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M3 - Article

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JO - Molecules and Cells

JF - Molecules and Cells

IS - 3

ER -

Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Abstract

Keywords

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