Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Eun Jung Chung; Young Kwan Sung; Mohammad Farooq; Younghee Kim; Sanguk Im; Won Young Tak; Yoon Jin Hwang; Yang Il Kim; Hyung Soo Han; Jung Chul Kim; Moon Kyu Kim

doi:10.1016/s1016-8478(23)15119-3

Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Eun Jung Chung
, Young Kwan Sung
, Mohammad Farooq
, Younghee Kim
, Sanguk Im
, Won Young Tak
, Yoon Jin Hwang
, Yang Il Kim
, Hyung Soo Han
, Jung Chul Kim
, Moon Kyu Kim

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

Original language	English
Pages (from-to)	382-387
Number of pages	6
Journal	Molecules and Cells
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/s1016-8478(23)15119-3
State	Published - Dec 2002

Keywords

Cluster
Hepatocellular Carcinoma
RT-PCR
cDNA Microarray

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10.1016/s1016-8478(23)15119-3

Cite this

@article{2e34d1fde25740f29c867992bd449517,

title = "Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray",

abstract = "We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.",

keywords = "Cluster, Hepatocellular Carcinoma, RT-PCR, cDNA Microarray",

author = "Chung, \{Eun Jung\} and Sung, \{Young Kwan\} and Mohammad Farooq and Younghee Kim and Sanguk Im and Tak, \{Won Young\} and Hwang, \{Yoon Jin\} and Kim, \{Yang Il\} and Han, \{Hyung Soo\} and Kim, \{Jung Chul\} and Kim, \{Moon Kyu\}",

year = "2002",

month = dec,

doi = "10.1016/s1016-8478(23)15119-3",

language = "English",

volume = "14",

pages = "382--387",

journal = "Molecules and Cells",

issn = "1016-8478",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

AU - Chung, Eun Jung

AU - Sung, Young Kwan

AU - Farooq, Mohammad

AU - Kim, Younghee

AU - Im, Sanguk

AU - Tak, Won Young

AU - Hwang, Yoon Jin

AU - Kim, Yang Il

AU - Han, Hyung Soo

AU - Kim, Jung Chul

AU - Kim, Moon Kyu

PY - 2002/12

Y1 - 2002/12

N2 - We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

AB - We performed gene expression profiling of normal and hepatocellular carcinoma (HCC) liver tissues using a high-density microarray that contained 3,063 human cDNA. The results of a microarray hybridization experiment from eight different HCC tissues were analyzed and classified by the Cluster program. Among these differentially-expressed genes, the galectin-3, serine/threonine kinase SGK, translation factor eIF-4A, -4B, -3, fibroblast growth factor receptor, and ribosomal protein L35A were up-regulated; the mRNAs of Nip3, decorin, and the insulin-like growth factor binding protein-3 were down-regulated in HCC. The differential expression of these genes was further confirmed by an RT-PCR analysis. In addition, our data suggest that the gene expression profile of HCC varies according to the histological types.

KW - Cluster

KW - Hepatocellular Carcinoma

KW - RT-PCR

KW - cDNA Microarray

UR - https://www.scopus.com/pages/publications/0037567661

U2 - 10.1016/s1016-8478(23)15119-3

DO - 10.1016/s1016-8478(23)15119-3

M3 - Article

C2 - 12521301

AN - SCOPUS:0037567661

SN - 1016-8478

VL - 14

SP - 382

EP - 387

JO - Molecules and Cells

JF - Molecules and Cells

IS - 3

ER -

Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray

Abstract

Keywords

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