Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

Hee Young Yang; Kee Jeong Dong; Seok Ho Kim; Kyoung Jin Chung; Eun Jin Cho; Hao Jin Cheng; Ung Yang; Ryeul Lee Sang; Dong Seok Lee; Tae Hoon Lee

doi:10.1002/jcb.21620

Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

Hee Young Yang, Kee Jeong Dong, Seok Ho Kim, Kyoung Jin Chung, Eun Jin Cho, Hao Jin Cheng, Ung Yang, Ryeul Lee Sang, Dong Seok Lee, Tae Hoon Lee

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Peroxiredoxin II knockout (Prdx II^-/-) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119 ⁺CD71⁺ cells increased in Prdx II^-/- mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II^+/+ and Prdx II ^-/- mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II^-/- mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34⁺ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.

Original language	English
Pages (from-to)	295-303
Number of pages	9
Journal	Journal of Cellular Biochemistry
Volume	104
Issue number	1
DOIs	https://doi.org/10.1002/jcb.21620
State	Published - 1 May 2008

Keywords

cDNA microarray
Erythropoiesis
Hematopoietic stem cell
Nucleotide binding protein
Prdx II knockout mice

Access to Document

10.1002/jcb.21620

Cite this

@article{06e67faaa69646d5ab24878309f82559,

title = "Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells",

abstract = "Peroxiredoxin II knockout (Prdx II-/-) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119 +CD71+ cells increased in Prdx II-/- mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II+/+ and Prdx II -/- mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II-/- mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34+ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.",

keywords = "cDNA microarray, Erythropoiesis, Hematopoietic stem cell, Nucleotide binding protein, Prdx II knockout mice",

author = "Yang, {Hee Young} and Dong, {Kee Jeong} and Kim, {Seok Ho} and Chung, {Kyoung Jin} and Cho, {Eun Jin} and Cheng, {Hao Jin} and Ung Yang and Sang, {Ryeul Lee} and Lee, {Dong Seok} and Lee, {Tae Hoon}",

year = "2008",

month = may,

day = "1",

doi = "10.1002/jcb.21620",

language = "English",

volume = "104",

pages = "295--303",

journal = "Journal of Cellular Biochemistry",

issn = "0730-2312",

publisher = "Wiley-Liss Inc.",

number = "1",

}

TY - JOUR

T1 - Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

AU - Yang, Hee Young

AU - Dong, Kee Jeong

AU - Kim, Seok Ho

AU - Chung, Kyoung Jin

AU - Cho, Eun Jin

AU - Cheng, Hao Jin

AU - Yang, Ung

AU - Sang, Ryeul Lee

AU - Lee, Dong Seok

AU - Lee, Tae Hoon

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Peroxiredoxin II knockout (Prdx II-/-) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119 +CD71+ cells increased in Prdx II-/- mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II+/+ and Prdx II -/- mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II-/- mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34+ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.

AB - Peroxiredoxin II knockout (Prdx II-/-) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119 +CD71+ cells increased in Prdx II-/- mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II+/+ and Prdx II -/- mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II-/- mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34+ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.

KW - cDNA microarray

KW - Erythropoiesis

KW - Hematopoietic stem cell

KW - Nucleotide binding protein

KW - Prdx II knockout mice

UR - http://www.scopus.com/inward/record.url?scp=46449126937&partnerID=8YFLogxK

U2 - 10.1002/jcb.21620

DO - 10.1002/jcb.21620

M3 - Article

C2 - 17990289

AN - SCOPUS:46449126937

SN - 0730-2312

VL - 104

SP - 295

EP - 303

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 1

ER -

Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this