Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

Peroxiredoxin II knockout (Prdx II^-/-) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter-119 ⁺CD71⁺ cells increased in Prdx II^-/- mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II^+/+ and Prdx II ^-/- mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up-regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp-127 were up-regulated during erythropoiesis in Prdx II^-/- mice. Among the six candidates, eIF3-p44, hnRNPH1, G3bp, and Zfpm-1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34⁺ cells. However, DJ-1 and Rbm3 were slightly increased only at day 12. Our results suggest that up-regulated NBPs might be involved during erythropoietic differentiation.