General Liquid-crystal droplets produced by microfluidics for urea detection

Mashooq Khan; Soo Young Park

doi:10.1016/j.snb.2014.05.115

General Liquid-crystal droplets produced by microfluidics for urea detection

Mashooq Khan, Soo Young Park

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

pH-sensitive 4-cyano-4′-pentylbiphenyl (5CB) droplets were produced for urea detection by coating with poly(acrylicacid-b-4-cyanobiphenyl-4- oxyundecylacrylate) (PAA-b-LCP) and covalently immobilizing urease on the PAA chains on the 5CB droplets using microfluidics. The functionalized 5CB droplets detected urea based on a bipolar-to-radial orientational change of the droplets observed by polarized optical microscopy using crossed polarizers. The detection limit was as low as 3 mM with a response time of 180 s. Urine was evaluated as a real sample for urea prescreening. This new and sensitive liquid crystal droplet-based urea biosensor has the merits of facile visual detection and is prospectively useful for urea prescreening for human subjects.

Original language	English
Pages (from-to)	516-522
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	202
DOIs	https://doi.org/10.1016/j.snb.2014.05.115
State	Published - 31 Oct 2014

Keywords

5CB
Biosensor
Liquid-crystal
PAA
Urea
Urease

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10.1016/j.snb.2014.05.115

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title = "General Liquid-crystal droplets produced by microfluidics for urea detection",

abstract = "pH-sensitive 4-cyano-4′-pentylbiphenyl (5CB) droplets were produced for urea detection by coating with poly(acrylicacid-b-4-cyanobiphenyl-4- oxyundecylacrylate) (PAA-b-LCP) and covalently immobilizing urease on the PAA chains on the 5CB droplets using microfluidics. The functionalized 5CB droplets detected urea based on a bipolar-to-radial orientational change of the droplets observed by polarized optical microscopy using crossed polarizers. The detection limit was as low as 3 mM with a response time of 180 s. Urine was evaluated as a real sample for urea prescreening. This new and sensitive liquid crystal droplet-based urea biosensor has the merits of facile visual detection and is prospectively useful for urea prescreening for human subjects.",

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AU - Park, Soo Young

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AB - pH-sensitive 4-cyano-4′-pentylbiphenyl (5CB) droplets were produced for urea detection by coating with poly(acrylicacid-b-4-cyanobiphenyl-4- oxyundecylacrylate) (PAA-b-LCP) and covalently immobilizing urease on the PAA chains on the 5CB droplets using microfluidics. The functionalized 5CB droplets detected urea based on a bipolar-to-radial orientational change of the droplets observed by polarized optical microscopy using crossed polarizers. The detection limit was as low as 3 mM with a response time of 180 s. Urine was evaluated as a real sample for urea prescreening. This new and sensitive liquid crystal droplet-based urea biosensor has the merits of facile visual detection and is prospectively useful for urea prescreening for human subjects.

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JO - Sensors and Actuators, B: Chemical

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General Liquid-crystal droplets produced by microfluidics for urea detection

Abstract

Keywords

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