Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries

Eun Mi Lee; Ah Young Kim; Eun Joo Lee; Jin Kyu Park; Se Il Park; Ssang Goo Cho; Hong Kyun Kim; Shin Yoon Kim; Kyu Shik Jeong

doi:10.3727/096368916X691691

Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries

Eun Mi Lee, Ah Young Kim, Eun Joo Lee, Jin Kyu Park, Se Il Park, Ssang Goo Cho, Hong Kyun Kim, Shin Yoon Kim, Kyu Shik Jeong

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.

Original language	English
Pages (from-to)	2003-2016
Number of pages	14
Journal	Cell Transplantation
Volume	25
Issue number	11
DOIs	https://doi.org/10.3727/096368916X691691
State	Published - 2016

Keywords

Horse
Induced pluripotent stem cells (iPSCs)
Muscle regeneration
Transplantation

Access to Document

10.3727/096368916X691691

Cite this

@article{4060089cf021411e85672dc87567fcb7,

title = "Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries",

abstract = "Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.",

keywords = "Horse, Induced pluripotent stem cells (iPSCs), Muscle regeneration, Transplantation",

author = "Lee, {Eun Mi} and Kim, {Ah Young} and Lee, {Eun Joo} and Park, {Jin Kyu} and Park, {Se Il} and Cho, {Ssang Goo} and Kim, {Hong Kyun} and Kim, {Shin Yoon} and Jeong, {Kyu Shik}",

note = "Publisher Copyright: {\textcopyright} 2016 Cognizant, LLC.",

year = "2016",

doi = "10.3727/096368916X691691",

language = "English",

volume = "25",

pages = "2003--2016",

journal = "Cell Transplantation",

issn = "0963-6897",

publisher = "SAGE Publications Inc.",

number = "11",

}

TY - JOUR

T1 - Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries

AU - Lee, Eun Mi

AU - Kim, Ah Young

AU - Lee, Eun Joo

AU - Park, Jin Kyu

AU - Park, Se Il

AU - Cho, Ssang Goo

AU - Kim, Hong Kyun

AU - Kim, Shin Yoon

AU - Jeong, Kyu Shik

PY - 2016

Y1 - 2016

N2 - Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.

AB - Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.

KW - Horse

KW - Induced pluripotent stem cells (iPSCs)

KW - Muscle regeneration

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=84994786423&partnerID=8YFLogxK

U2 - 10.3727/096368916X691691

DO - 10.3727/096368916X691691

M3 - Article

C2 - 27226077

AN - SCOPUS:84994786423

SN - 0963-6897

VL - 25

SP - 2003

EP - 2016

JO - Cell Transplantation

JF - Cell Transplantation

IS - 11

ER -

Generation of equine-induced pluripotent stem cells and analysis of their therapeutic potential for muscle injuries

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this